Cell proliferation and interleukin-6-type cytokine signaling are implicated by gene expression responses in early optic nerve head injury in rat glaucoma

Elaine Johnson, Thomas A. Doser, William O. Cepurna, Jennifer A. Dyck, Lijun Jia, Ying Guo, Wendi S. Lambert, John Morrison

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

PURPOSE. In glaucoma, the optic nerve head (ONH) is the principal site of initial axonal injury, and elevated intraocular pressure (IOP) is the predominant risk factor. However, the initial responses of the ONH to elevated IOP are unknown. Here the authors use a rat glaucoma model to characterize ONH gene expression changes associated with early optic nerve injury. METHODS. Unilateral IOP elevation was produced in rats by episcleral vein injection of hypertonic saline. ONH mRNA was extracted, and retrobulbar optic nerve cross-sections were graded for axonal degeneration. Gene expression was determined by microarray and quantitative PCR (QPCR) analysis. Significantly altered gene expression was determined by multiclass analysis and ANOVA. DAVID gene ontology determined the functional categories of significantly affected genes. RESULTS. The Early Injury group consisted of ONH from eyes with

Original languageEnglish (US)
Pages (from-to)504-518
Number of pages15
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number1
DOIs
StatePublished - Jan 2011

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Optic Nerve Injuries
Optic Disk
Craniocerebral Trauma
Glaucoma
Interleukin-6
Cell Proliferation
Cytokines
Gene Expression
Intraocular Pressure
Gene Ontology
Wounds and Injuries
Optic Nerve
Veins
Analysis of Variance
Polymerase Chain Reaction
Messenger RNA
Injections
Genes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Cell proliferation and interleukin-6-type cytokine signaling are implicated by gene expression responses in early optic nerve head injury in rat glaucoma. / Johnson, Elaine; Doser, Thomas A.; Cepurna, William O.; Dyck, Jennifer A.; Jia, Lijun; Guo, Ying; Lambert, Wendi S.; Morrison, John.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 1, 01.2011, p. 504-518.

Research output: Contribution to journalArticle

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AU - Jia, Lijun

AU - Guo, Ying

AU - Lambert, Wendi S.

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