Cell cycle features of primate embryonic stem cells

Anne Catherine Fluckiger, Guillaume Marcy, Mélanie Marchand, Didier Négre, François Loïc Cosset, Shoukhrat Mitalipov, Don Wolf, Pierre Savatier, Colette Dehay

    Research output: Contribution to journalArticle

    142 Citations (Scopus)

    Abstract

    Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G1 after γ-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G1 phase are reduced or absent.

    Original languageEnglish (US)
    Pages (from-to)547-556
    Number of pages10
    JournalStem Cells
    Volume24
    Issue number3
    DOIs
    StatePublished - Mar 2006

    Fingerprint

    Embryonic Stem Cells
    Primates
    Cell Cycle
    Mitogen-Activated Protein Kinase Kinases
    G1 Phase
    Macaca mulatta
    Cyclin E
    Growth
    Cell Cycle Checkpoints
    Haplorhini
    Flow Cytometry
    Pharmacology
    Serum

    Keywords

    • Cell cycle
    • Rhesus embryonic stem cells
    • Stemness

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Fluckiger, A. C., Marcy, G., Marchand, M., Négre, D., Cosset, F. L., Mitalipov, S., ... Dehay, C. (2006). Cell cycle features of primate embryonic stem cells. Stem Cells, 24(3), 547-556. https://doi.org/10.1634/stemcells.2005-0194

    Cell cycle features of primate embryonic stem cells. / Fluckiger, Anne Catherine; Marcy, Guillaume; Marchand, Mélanie; Négre, Didier; Cosset, François Loïc; Mitalipov, Shoukhrat; Wolf, Don; Savatier, Pierre; Dehay, Colette.

    In: Stem Cells, Vol. 24, No. 3, 03.2006, p. 547-556.

    Research output: Contribution to journalArticle

    Fluckiger, AC, Marcy, G, Marchand, M, Négre, D, Cosset, FL, Mitalipov, S, Wolf, D, Savatier, P & Dehay, C 2006, 'Cell cycle features of primate embryonic stem cells', Stem Cells, vol. 24, no. 3, pp. 547-556. https://doi.org/10.1634/stemcells.2005-0194
    Fluckiger AC, Marcy G, Marchand M, Négre D, Cosset FL, Mitalipov S et al. Cell cycle features of primate embryonic stem cells. Stem Cells. 2006 Mar;24(3):547-556. https://doi.org/10.1634/stemcells.2005-0194
    Fluckiger, Anne Catherine ; Marcy, Guillaume ; Marchand, Mélanie ; Négre, Didier ; Cosset, François Loïc ; Mitalipov, Shoukhrat ; Wolf, Don ; Savatier, Pierre ; Dehay, Colette. / Cell cycle features of primate embryonic stem cells. In: Stem Cells. 2006 ; Vol. 24, No. 3. pp. 547-556.
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