Categorial selection of the antibody repertoire in splenic B cells

Robert Schelonka, Jason Tanner, Yingxin Zhuang, G. Larry Gartland, Michael Zemlin, Harry W. Schroeder

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.

Original languageEnglish (US)
Pages (from-to)1010-1021
Number of pages12
JournalEuropean Journal of Immunology
Volume37
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

B-Lymphocytes
Antibodies
Bone Marrow
Bone Marrow Cells
B-Lymphocyte Subsets
Immunoglobulin D
Immunoglobulin Heavy Chains
Amino Acid Motifs
Immunoglobulin M
Arginine
Spleen
Binding Sites
Antigens
Amino Acids

Keywords

  • Antibodies
  • B cells
  • Repertoire development
  • Rodent
  • Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Schelonka, R., Tanner, J., Zhuang, Y., Gartland, G. L., Zemlin, M., & Schroeder, H. W. (2007). Categorial selection of the antibody repertoire in splenic B cells. European Journal of Immunology, 37(4), 1010-1021. https://doi.org/10.1002/eji.200636569

Categorial selection of the antibody repertoire in splenic B cells. / Schelonka, Robert; Tanner, Jason; Zhuang, Yingxin; Gartland, G. Larry; Zemlin, Michael; Schroeder, Harry W.

In: European Journal of Immunology, Vol. 37, No. 4, 04.2007, p. 1010-1021.

Research output: Contribution to journalArticle

Schelonka, R, Tanner, J, Zhuang, Y, Gartland, GL, Zemlin, M & Schroeder, HW 2007, 'Categorial selection of the antibody repertoire in splenic B cells', European Journal of Immunology, vol. 37, no. 4, pp. 1010-1021. https://doi.org/10.1002/eji.200636569
Schelonka, Robert ; Tanner, Jason ; Zhuang, Yingxin ; Gartland, G. Larry ; Zemlin, Michael ; Schroeder, Harry W. / Categorial selection of the antibody repertoire in splenic B cells. In: European Journal of Immunology. 2007 ; Vol. 37, No. 4. pp. 1010-1021.
@article{6ae1ec61541f4fe1903b67562b7c368f,
title = "Categorial selection of the antibody repertoire in splenic B cells",
abstract = "In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.",
keywords = "Antibodies, B cells, Repertoire development, Rodent, Spleen",
author = "Robert Schelonka and Jason Tanner and Yingxin Zhuang and Gartland, {G. Larry} and Michael Zemlin and Schroeder, {Harry W.}",
year = "2007",
month = "4",
doi = "10.1002/eji.200636569",
language = "English (US)",
volume = "37",
pages = "1010--1021",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Categorial selection of the antibody repertoire in splenic B cells

AU - Schelonka, Robert

AU - Tanner, Jason

AU - Zhuang, Yingxin

AU - Gartland, G. Larry

AU - Zemlin, Michael

AU - Schroeder, Harry W.

PY - 2007/4

Y1 - 2007/4

N2 - In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.

AB - In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.

KW - Antibodies

KW - B cells

KW - Repertoire development

KW - Rodent

KW - Spleen

UR - http://www.scopus.com/inward/record.url?scp=34248209055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248209055&partnerID=8YFLogxK

U2 - 10.1002/eji.200636569

DO - 10.1002/eji.200636569

M3 - Article

VL - 37

SP - 1010

EP - 1021

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 4

ER -