TY - JOUR
T1 - Categorial selection of the antibody repertoire in splenic B cells
AU - Schelonka, Robert L.
AU - Tanner, Jason
AU - Zhuang, Yingxin
AU - Gartland, G. Larry
AU - Zemlin, Michael
AU - Schroeder, Harry W.
PY - 2007/4
Y1 - 2007/4
N2 - In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.
AB - In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.
KW - Antibodies
KW - B cells
KW - Repertoire development
KW - Rodent
KW - Spleen
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U2 - 10.1002/eji.200636569
DO - 10.1002/eji.200636569
M3 - Article
C2 - 17345580
AN - SCOPUS:34248209055
SN - 0014-2980
VL - 37
SP - 1010
EP - 1021
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -