Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules

Kristen J. Verhey, Debra Meyer, Reneé Deehan, John Blenis, Bruce Schnapp, Tom A. Rapoport, Ben Margolis

Research output: Contribution to journalArticle

464 Citations (Scopus)

Abstract

The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide repeat (TPR) motifs. Three proteins were found, the c-jun NH2-terminal kinase (JNK)-interacting proteins (JIPs) JIP-1, JIP-2, and JIP-3, which are scaffolding proteins for the JNK signaling pathway. Concentration of JIPs in nerve terminals requires kinesin, as evident from the analysis of JIP COOH-terminal mutants and dominant negative kinesin constructs. Coprecipitation experiments suggest that kinesin carries the JIP scaffolds preloaded with cytoplasmic (dual leucine zipper-bearing kinase) and transmembrane signaling molecules (the Reelin receptor, ApoER2). These results demonstrate a direct interaction between conventional kinesin and a cargo, indicate that motor proteins are linked to their membranous cargo via scaffolding proteins, and support a role for motor proteins in spatial regulation of signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)959-970
Number of pages12
JournalJournal of Cell Biology
Volume152
Issue number5
DOIs
StatePublished - Mar 5 2001
Externally publishedYes

Fingerprint

Kinesin
Proteins
JNK Mitogen-Activated Protein Kinases
Microtubules
Signal Transduction

Keywords

  • JNK signaling
  • Kinesin
  • Molecular motors
  • Reelin
  • Scaffolding protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Verhey, K. J., Meyer, D., Deehan, R., Blenis, J., Schnapp, B., Rapoport, T. A., & Margolis, B. (2001). Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. Journal of Cell Biology, 152(5), 959-970. https://doi.org/10.1083/jcb.152.5.959

Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. / Verhey, Kristen J.; Meyer, Debra; Deehan, Reneé; Blenis, John; Schnapp, Bruce; Rapoport, Tom A.; Margolis, Ben.

In: Journal of Cell Biology, Vol. 152, No. 5, 05.03.2001, p. 959-970.

Research output: Contribution to journalArticle

Verhey, KJ, Meyer, D, Deehan, R, Blenis, J, Schnapp, B, Rapoport, TA & Margolis, B 2001, 'Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules', Journal of Cell Biology, vol. 152, no. 5, pp. 959-970. https://doi.org/10.1083/jcb.152.5.959
Verhey, Kristen J. ; Meyer, Debra ; Deehan, Reneé ; Blenis, John ; Schnapp, Bruce ; Rapoport, Tom A. ; Margolis, Ben. / Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. In: Journal of Cell Biology. 2001 ; Vol. 152, No. 5. pp. 959-970.
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