Cardiovascular regulation after destruction of the C1 cell group of the rostral ventrolateral medulla in rats

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

To evaluate the role of C1 neurons in the rostral ventrolateral medulla (RVLM) in cardiovascular regulation, we studied rats in which this cell group was destroyed by the injection of anti-dopamine-β-hydroxylase-saporin into the RVLM. These immunotoxin injections resulted in 32-99% depletion of the RVLM-C1 neurons and ∼50% depletion of the A5 cell population. In conscious rats with large (>80%) depletion of the RVLM-C1 cell population, resting arterial pressure was ∼10 mmHg lower than in control injected rats, although heart rate was not significantly different. Similar results were observed when arterial pressure was recorded in urethan-anesthetized rats, although under anesthesia, heart rate was also reduced in rats with large (>80%) depletion of the RVLM-C1 neuronal population. Sympathoexcitatory responses to baroreceptor unloading, chemoreceptor activation, and electrical stimulation of sciatic nerve afferent fibers were attenuated in rats with >80% depletion of the RVLM-C1 cell population. These effects of RVLM-C1 plus A5 cell populations were not mimicked by either smaller lesions of the RVLM-C1 population or by selective destruction of the A5 cell population with 6-hydroxydopamine. Sympathoinhibitory responses such as decreases in arterial pressure and heart rate evoked by injection of GABA into the RVLM or by intravenous phenylbiguanide administration were not altered by RVLM-C1 plus A5 cell depletion. These data suggest that RVLM-C1 cells contribute to the maintenance of baseline arterial pressure and play an integral role in sympatho-excitatory responses.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume285
Issue number6 54-6
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Arterial Pressure
Population
Heart Rate
Injections
Neurons
Immunotoxins
Pressoreceptors
Oxidopamine
Urethane
Sciatic Nerve
Mixed Function Oxygenases
Nerve Fibers
Intravenous Administration
gamma-Aminobutyric Acid
Electric Stimulation
Dopamine
Anesthesia
Maintenance

Keywords

  • Baroreceptor reflex
  • Chemoreceptor reflex
  • Immunotoxin
  • Phenylethanolamine-N-methyltransferase
  • Somatic pressor reflex

ASJC Scopus subject areas

  • Physiology

Cite this

@article{021ace2f35314923afab6b7208363a8c,
title = "Cardiovascular regulation after destruction of the C1 cell group of the rostral ventrolateral medulla in rats",
abstract = "To evaluate the role of C1 neurons in the rostral ventrolateral medulla (RVLM) in cardiovascular regulation, we studied rats in which this cell group was destroyed by the injection of anti-dopamine-β-hydroxylase-saporin into the RVLM. These immunotoxin injections resulted in 32-99{\%} depletion of the RVLM-C1 neurons and ∼50{\%} depletion of the A5 cell population. In conscious rats with large (>80{\%}) depletion of the RVLM-C1 cell population, resting arterial pressure was ∼10 mmHg lower than in control injected rats, although heart rate was not significantly different. Similar results were observed when arterial pressure was recorded in urethan-anesthetized rats, although under anesthesia, heart rate was also reduced in rats with large (>80{\%}) depletion of the RVLM-C1 neuronal population. Sympathoexcitatory responses to baroreceptor unloading, chemoreceptor activation, and electrical stimulation of sciatic nerve afferent fibers were attenuated in rats with >80{\%} depletion of the RVLM-C1 cell population. These effects of RVLM-C1 plus A5 cell populations were not mimicked by either smaller lesions of the RVLM-C1 population or by selective destruction of the A5 cell population with 6-hydroxydopamine. Sympathoinhibitory responses such as decreases in arterial pressure and heart rate evoked by injection of GABA into the RVLM or by intravenous phenylbiguanide administration were not altered by RVLM-C1 plus A5 cell depletion. These data suggest that RVLM-C1 cells contribute to the maintenance of baseline arterial pressure and play an integral role in sympatho-excitatory responses.",
keywords = "Baroreceptor reflex, Chemoreceptor reflex, Immunotoxin, Phenylethanolamine-N-methyltransferase, Somatic pressor reflex",
author = "Madden, {Christopher (Chris)} and Sved, {Alan F.}",
year = "2003",
month = "12",
language = "English (US)",
volume = "285",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 54-6",

}

TY - JOUR

T1 - Cardiovascular regulation after destruction of the C1 cell group of the rostral ventrolateral medulla in rats

AU - Madden, Christopher (Chris)

AU - Sved, Alan F.

PY - 2003/12

Y1 - 2003/12

N2 - To evaluate the role of C1 neurons in the rostral ventrolateral medulla (RVLM) in cardiovascular regulation, we studied rats in which this cell group was destroyed by the injection of anti-dopamine-β-hydroxylase-saporin into the RVLM. These immunotoxin injections resulted in 32-99% depletion of the RVLM-C1 neurons and ∼50% depletion of the A5 cell population. In conscious rats with large (>80%) depletion of the RVLM-C1 cell population, resting arterial pressure was ∼10 mmHg lower than in control injected rats, although heart rate was not significantly different. Similar results were observed when arterial pressure was recorded in urethan-anesthetized rats, although under anesthesia, heart rate was also reduced in rats with large (>80%) depletion of the RVLM-C1 neuronal population. Sympathoexcitatory responses to baroreceptor unloading, chemoreceptor activation, and electrical stimulation of sciatic nerve afferent fibers were attenuated in rats with >80% depletion of the RVLM-C1 cell population. These effects of RVLM-C1 plus A5 cell populations were not mimicked by either smaller lesions of the RVLM-C1 population or by selective destruction of the A5 cell population with 6-hydroxydopamine. Sympathoinhibitory responses such as decreases in arterial pressure and heart rate evoked by injection of GABA into the RVLM or by intravenous phenylbiguanide administration were not altered by RVLM-C1 plus A5 cell depletion. These data suggest that RVLM-C1 cells contribute to the maintenance of baseline arterial pressure and play an integral role in sympatho-excitatory responses.

AB - To evaluate the role of C1 neurons in the rostral ventrolateral medulla (RVLM) in cardiovascular regulation, we studied rats in which this cell group was destroyed by the injection of anti-dopamine-β-hydroxylase-saporin into the RVLM. These immunotoxin injections resulted in 32-99% depletion of the RVLM-C1 neurons and ∼50% depletion of the A5 cell population. In conscious rats with large (>80%) depletion of the RVLM-C1 cell population, resting arterial pressure was ∼10 mmHg lower than in control injected rats, although heart rate was not significantly different. Similar results were observed when arterial pressure was recorded in urethan-anesthetized rats, although under anesthesia, heart rate was also reduced in rats with large (>80%) depletion of the RVLM-C1 neuronal population. Sympathoexcitatory responses to baroreceptor unloading, chemoreceptor activation, and electrical stimulation of sciatic nerve afferent fibers were attenuated in rats with >80% depletion of the RVLM-C1 cell population. These effects of RVLM-C1 plus A5 cell populations were not mimicked by either smaller lesions of the RVLM-C1 population or by selective destruction of the A5 cell population with 6-hydroxydopamine. Sympathoinhibitory responses such as decreases in arterial pressure and heart rate evoked by injection of GABA into the RVLM or by intravenous phenylbiguanide administration were not altered by RVLM-C1 plus A5 cell depletion. These data suggest that RVLM-C1 cells contribute to the maintenance of baseline arterial pressure and play an integral role in sympatho-excitatory responses.

KW - Baroreceptor reflex

KW - Chemoreceptor reflex

KW - Immunotoxin

KW - Phenylethanolamine-N-methyltransferase

KW - Somatic pressor reflex

UR - http://www.scopus.com/inward/record.url?scp=0344009500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344009500&partnerID=8YFLogxK

M3 - Article

C2 - 12933337

AN - SCOPUS:0344009500

VL - 285

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 6 54-6

ER -