Can we see PIP 3 and hydrogen peroxide with a single probe?

Natalie M. Mishina, Ivan Bogeski, Dmitry A. Bolotin, Markus Hoth, Barbara A. Niemeyer, Carsten Schultz, Eleva V. Zagaynova, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journalShort surveypeer-review

18 Scopus citations


A genetically encoded sensor for parallel measurements of phosphatidylinositol 3-kinase activity and hydrogen peroxide (H 2O 2) levels (termed PIP-SHOW) was developed. Upon elevation of local phosphatidylinositol 3,4,5-trisphosphate (PIP 3) concentration, the sensor translocates from the cytosol to the plasma membrane, while a ratiometric excitation change rapidly and simultaneously reports changes in the concentration of H 2O 2. The dynamics of PIP 3 and H 2O 2 generation were monitored in platelet-derived growth factor-stimulated fibroblasts and in T-lymphocytes after formation of an immunological synapse. We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts.

Original languageEnglish (US)
Pages (from-to)505-512
Number of pages8
JournalAntioxidants and Redox Signaling
Issue number3
StatePublished - Aug 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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