Can we see PIP 3 and hydrogen peroxide with a single probe?

Natalie M. Mishina, Ivan Bogeski, Dmitry A. Bolotin, Markus Hoth, Barbara A. Niemeyer, Carsten Schultz, Eleva V. Zagaynova, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journalShort survey

15 Citations (Scopus)

Abstract

A genetically encoded sensor for parallel measurements of phosphatidylinositol 3-kinase activity and hydrogen peroxide (H 2O 2) levels (termed PIP-SHOW) was developed. Upon elevation of local phosphatidylinositol 3,4,5-trisphosphate (PIP 3) concentration, the sensor translocates from the cytosol to the plasma membrane, while a ratiometric excitation change rapidly and simultaneously reports changes in the concentration of H 2O 2. The dynamics of PIP 3 and H 2O 2 generation were monitored in platelet-derived growth factor-stimulated fibroblasts and in T-lymphocytes after formation of an immunological synapse. We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts.

Original languageEnglish (US)
Pages (from-to)505-512
Number of pages8
JournalAntioxidants and Redox Signaling
Volume17
Issue number3
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

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Immunological Synapses
Phosphatidylinositol 3-Kinase
Platelet-Derived Growth Factor
Cytosol
Hydrogen Peroxide
Fibroblasts
Cell Membrane
T-Lymphocytes
Sensors
T-cells
Cell membranes
phosphatidylinositol 3,4,5-triphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Mishina, N. M., Bogeski, I., Bolotin, D. A., Hoth, M., Niemeyer, B. A., Schultz, C., ... Belousov, V. V. (2012). Can we see PIP 3 and hydrogen peroxide with a single probe? Antioxidants and Redox Signaling, 17(3), 505-512. https://doi.org/10.1089/ars.2012.4574

Can we see PIP 3 and hydrogen peroxide with a single probe? / Mishina, Natalie M.; Bogeski, Ivan; Bolotin, Dmitry A.; Hoth, Markus; Niemeyer, Barbara A.; Schultz, Carsten; Zagaynova, Eleva V.; Lukyanov, Sergey; Belousov, Vsevolod V.

In: Antioxidants and Redox Signaling, Vol. 17, No. 3, 01.08.2012, p. 505-512.

Research output: Contribution to journalShort survey

Mishina, NM, Bogeski, I, Bolotin, DA, Hoth, M, Niemeyer, BA, Schultz, C, Zagaynova, EV, Lukyanov, S & Belousov, VV 2012, 'Can we see PIP 3 and hydrogen peroxide with a single probe?', Antioxidants and Redox Signaling, vol. 17, no. 3, pp. 505-512. https://doi.org/10.1089/ars.2012.4574
Mishina, Natalie M. ; Bogeski, Ivan ; Bolotin, Dmitry A. ; Hoth, Markus ; Niemeyer, Barbara A. ; Schultz, Carsten ; Zagaynova, Eleva V. ; Lukyanov, Sergey ; Belousov, Vsevolod V. / Can we see PIP 3 and hydrogen peroxide with a single probe?. In: Antioxidants and Redox Signaling. 2012 ; Vol. 17, No. 3. pp. 505-512.
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