Calibration of optimal use parameters for an ultraviolet light-emitting diode in eliminating bacterial contamination on needleless connectors

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims: Needleless connectors may develop bacterial contamination and cause central-line-associated bloodstream infections (CLABSI) despite rigorous application of best-practice. Ultraviolet (UV) light-emitting diodes (LED) are an emerging, increasingly affordable disinfection technology. We tested the hypothesis that a low-power UV LED could reliably eliminate bacteria on needleless central-line ports in a laboratory model of central-line contamination. Methods and Results: Needleless central-line connectors were inoculated with Staphylococcus aureus. A 285 nm UV LED was used in calibrated fashion to expose contaminated connectors. Ports were directly applied to agar plates and flushed with sterile saline, allowing assessment of bacterial survival on the port surface and in simulated usage flow-through fluid. UV applied to needleless central-line connectors was highly lethal at 0·5 cm distance at all tested exposure times. At distances >1·5 cm both simulated flow-through and port surface cultures demonstrated significant bacterial growth following UV exposure. Logarithmic-phase S. aureus subcultures were highly susceptible to UV induction/maintenance dosing. Conclusions: Low-power UV LED doses at fixed time and distance from needleless central-line connector ports reduced cultivable S. aureus from >106 CFU to below detectable levels in this laboratory simulation of central-line port contamination. Significance and Impact of the study: Low-power UV LEDs may represent a feasible alternative to current best-practice in connector decontamination.

Original languageEnglish (US)
Pages (from-to)1298-1305
Number of pages8
JournalJournal of Applied Microbiology
Volume118
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Ultraviolet Rays
Calibration
Practice Guidelines
Decontamination
Disinfection
S Phase
Agar
Staphylococcus aureus
Maintenance
Technology
Bacteria
Growth
Infection

Keywords

  • Central venous catheter
  • Central-line-associated bloodstream infections
  • Disinfection
  • Needleless connector
  • Ultraviolet light-emitting diode

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

@article{0bfe0cbcae9740a098a79ccf954bf3a2,
title = "Calibration of optimal use parameters for an ultraviolet light-emitting diode in eliminating bacterial contamination on needleless connectors",
abstract = "Aims: Needleless connectors may develop bacterial contamination and cause central-line-associated bloodstream infections (CLABSI) despite rigorous application of best-practice. Ultraviolet (UV) light-emitting diodes (LED) are an emerging, increasingly affordable disinfection technology. We tested the hypothesis that a low-power UV LED could reliably eliminate bacteria on needleless central-line ports in a laboratory model of central-line contamination. Methods and Results: Needleless central-line connectors were inoculated with Staphylococcus aureus. A 285 nm UV LED was used in calibrated fashion to expose contaminated connectors. Ports were directly applied to agar plates and flushed with sterile saline, allowing assessment of bacterial survival on the port surface and in simulated usage flow-through fluid. UV applied to needleless central-line connectors was highly lethal at 0·5 cm distance at all tested exposure times. At distances >1·5 cm both simulated flow-through and port surface cultures demonstrated significant bacterial growth following UV exposure. Logarithmic-phase S. aureus subcultures were highly susceptible to UV induction/maintenance dosing. Conclusions: Low-power UV LED doses at fixed time and distance from needleless central-line connector ports reduced cultivable S. aureus from >106 CFU to below detectable levels in this laboratory simulation of central-line port contamination. Significance and Impact of the study: Low-power UV LEDs may represent a feasible alternative to current best-practice in connector decontamination.",
keywords = "Central venous catheter, Central-line-associated bloodstream infections, Disinfection, Needleless connector, Ultraviolet light-emitting diode",
author = "Michael Hutchens and Drennan, {S. L.} and Eric Cambronne",
year = "2015",
month = "6",
day = "1",
doi = "10.1111/jam.12802",
language = "English (US)",
volume = "118",
pages = "1298--1305",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Calibration of optimal use parameters for an ultraviolet light-emitting diode in eliminating bacterial contamination on needleless connectors

AU - Hutchens, Michael

AU - Drennan, S. L.

AU - Cambronne, Eric

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Aims: Needleless connectors may develop bacterial contamination and cause central-line-associated bloodstream infections (CLABSI) despite rigorous application of best-practice. Ultraviolet (UV) light-emitting diodes (LED) are an emerging, increasingly affordable disinfection technology. We tested the hypothesis that a low-power UV LED could reliably eliminate bacteria on needleless central-line ports in a laboratory model of central-line contamination. Methods and Results: Needleless central-line connectors were inoculated with Staphylococcus aureus. A 285 nm UV LED was used in calibrated fashion to expose contaminated connectors. Ports were directly applied to agar plates and flushed with sterile saline, allowing assessment of bacterial survival on the port surface and in simulated usage flow-through fluid. UV applied to needleless central-line connectors was highly lethal at 0·5 cm distance at all tested exposure times. At distances >1·5 cm both simulated flow-through and port surface cultures demonstrated significant bacterial growth following UV exposure. Logarithmic-phase S. aureus subcultures were highly susceptible to UV induction/maintenance dosing. Conclusions: Low-power UV LED doses at fixed time and distance from needleless central-line connector ports reduced cultivable S. aureus from >106 CFU to below detectable levels in this laboratory simulation of central-line port contamination. Significance and Impact of the study: Low-power UV LEDs may represent a feasible alternative to current best-practice in connector decontamination.

AB - Aims: Needleless connectors may develop bacterial contamination and cause central-line-associated bloodstream infections (CLABSI) despite rigorous application of best-practice. Ultraviolet (UV) light-emitting diodes (LED) are an emerging, increasingly affordable disinfection technology. We tested the hypothesis that a low-power UV LED could reliably eliminate bacteria on needleless central-line ports in a laboratory model of central-line contamination. Methods and Results: Needleless central-line connectors were inoculated with Staphylococcus aureus. A 285 nm UV LED was used in calibrated fashion to expose contaminated connectors. Ports were directly applied to agar plates and flushed with sterile saline, allowing assessment of bacterial survival on the port surface and in simulated usage flow-through fluid. UV applied to needleless central-line connectors was highly lethal at 0·5 cm distance at all tested exposure times. At distances >1·5 cm both simulated flow-through and port surface cultures demonstrated significant bacterial growth following UV exposure. Logarithmic-phase S. aureus subcultures were highly susceptible to UV induction/maintenance dosing. Conclusions: Low-power UV LED doses at fixed time and distance from needleless central-line connector ports reduced cultivable S. aureus from >106 CFU to below detectable levels in this laboratory simulation of central-line port contamination. Significance and Impact of the study: Low-power UV LEDs may represent a feasible alternative to current best-practice in connector decontamination.

KW - Central venous catheter

KW - Central-line-associated bloodstream infections

KW - Disinfection

KW - Needleless connector

KW - Ultraviolet light-emitting diode

UR - http://www.scopus.com/inward/record.url?scp=84929519901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929519901&partnerID=8YFLogxK

U2 - 10.1111/jam.12802

DO - 10.1111/jam.12802

M3 - Article

C2 - 25801979

AN - SCOPUS:84929519901

VL - 118

SP - 1298

EP - 1305

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -