Calculation of gestational age in late second and third trimesters by ex vivo magnetic resonance spectroscopy of amniotic fluid

Brad R. Cohn, Emily Y. Fukuchi, Bonnie N. Joe, Mark G. Swanson, John Kurhanewicz, Jingwei Yu, Aaron Caughey

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: The purpose of this study was to develop a reliable model for the calculation of gestational age (GA) in second and third trimesters with the use of amniotic fluid (AF) metabolite profiles that were determined by magnetic resonance spectroscopy. Study Design: High-resolution (11.7 T) ex vivo magnetic resonance spectroscopy was performed on 95 AF samples (mean, 31.7 weeks; range, 15.6-39.9 weeks). GA was determined by last menstrual period or first-trimester ultrasound scanning. Concentrations of 21 AF metabolites were measured with automated techniques. Metabolite concentrations, inverses, natural logs, and squares were entered as predictive variables in a stepwise linear regression model. Results: The following formula was derived: GA = 64.922 - (14.456 × alanine) + (4.965 × natural log [creatinine]) - (0.931 × glucose) - (5.202 × valine). This model fit the data with an R2 value of 0.926. Average error among all samples was ±1.75 weeks (SD, ±1.43 weeks), for the second trimester was ±2.21 weeks (SD, ±1.78 weeks), and for the third trimester was ±1.59 weeks (SD, ±1.26 weeks). Conclusion: Statistical modeling accurately predicted GA with amniotic fluid metabolite profiles that were obtained by magnetic resonance spectroscopy, which may represent a significant improvement over conventional ultrasound dating in the third trimester. Future studies should compare these techniques directly.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number1
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Third Pregnancy Trimester
Second Pregnancy Trimester
Amniotic Fluid
Gestational Age
Magnetic Resonance Spectroscopy
Linear Models
Valine
First Pregnancy Trimester
Alanine
Creatinine
Glucose

Keywords

  • amniotic fluid
  • gestational age
  • magnetic resonance spectroscopy
  • multiple linear regression models

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Calculation of gestational age in late second and third trimesters by ex vivo magnetic resonance spectroscopy of amniotic fluid. / Cohn, Brad R.; Fukuchi, Emily Y.; Joe, Bonnie N.; Swanson, Mark G.; Kurhanewicz, John; Yu, Jingwei; Caughey, Aaron.

In: American Journal of Obstetrics and Gynecology, Vol. 203, No. 1, 07.2010.

Research output: Contribution to journalArticle

Cohn, Brad R. ; Fukuchi, Emily Y. ; Joe, Bonnie N. ; Swanson, Mark G. ; Kurhanewicz, John ; Yu, Jingwei ; Caughey, Aaron. / Calculation of gestational age in late second and third trimesters by ex vivo magnetic resonance spectroscopy of amniotic fluid. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 203, No. 1.
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