Abstract
There have been several independent observations over the last decade attesting to the association of deoxyribonucleic acid with the surface membranes of certain mammalian cells. In general these findings have generated little interest as it has usually been assumed that this association is a non-specific epiphenomenon. In this study, we show that the isolated cell membranes of human neutrophils possess a DNA binding molecule with a molecular weight of 30,000. The techniques used to demonstrate this DNA binding molecule, namely the use of biotinylated DNA to probe proteins that have been electrophoretically separated and blotted onto nitrocellulose, should find a general applicability in identifying putative cell surface receptors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1034-1039 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 122 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 16 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Biotin-labelled DNA : A novel approach for the recognition of a DNA binding site on cell membranes. / Gabor, Gary; Bennett, Robert (Rob).
In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1034-1039.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biotin-labelled DNA
T2 - A novel approach for the recognition of a DNA binding site on cell membranes
AU - Gabor, Gary
AU - Bennett, Robert (Rob)
PY - 1984/8/16
Y1 - 1984/8/16
N2 - There have been several independent observations over the last decade attesting to the association of deoxyribonucleic acid with the surface membranes of certain mammalian cells. In general these findings have generated little interest as it has usually been assumed that this association is a non-specific epiphenomenon. In this study, we show that the isolated cell membranes of human neutrophils possess a DNA binding molecule with a molecular weight of 30,000. The techniques used to demonstrate this DNA binding molecule, namely the use of biotinylated DNA to probe proteins that have been electrophoretically separated and blotted onto nitrocellulose, should find a general applicability in identifying putative cell surface receptors.
AB - There have been several independent observations over the last decade attesting to the association of deoxyribonucleic acid with the surface membranes of certain mammalian cells. In general these findings have generated little interest as it has usually been assumed that this association is a non-specific epiphenomenon. In this study, we show that the isolated cell membranes of human neutrophils possess a DNA binding molecule with a molecular weight of 30,000. The techniques used to demonstrate this DNA binding molecule, namely the use of biotinylated DNA to probe proteins that have been electrophoretically separated and blotted onto nitrocellulose, should find a general applicability in identifying putative cell surface receptors.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021765481&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(84)91195-1
DO - 10.1016/0006-291X(84)91195-1
M3 - Article
C2 - 6236803
AN - SCOPUS:0021765481
VL - 122
SP - 1034
EP - 1039
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -