Biotin-labelled DNA: A novel approach for the recognition of a DNA binding site on cell membranes

Gary Gabor, Robert M. Bennett

    Research output: Contribution to journalArticle

    26 Scopus citations

    Abstract

    There have been several independent observations over the last decade attesting to the association of deoxyribonucleic acid with the surface membranes of certain mammalian cells. In general these findings have generated little interest as it has usually been assumed that this association is a non-specific epiphenomenon. In this study, we show that the isolated cell membranes of human neutrophils possess a DNA binding molecule with a molecular weight of 30,000. The techniques used to demonstrate this DNA binding molecule, namely the use of biotinylated DNA to probe proteins that have been electrophoretically separated and blotted onto nitrocellulose, should find a general applicability in identifying putative cell surface receptors.

    Original languageEnglish (US)
    Pages (from-to)1034-1039
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume122
    Issue number3
    DOIs
    StatePublished - Aug 16 1984

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    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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