Biotin-labelled DNA: A novel approach for the recognition of a DNA binding site on cell membranes

Gary Gabor, Robert (Rob) Bennett

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

There have been several independent observations over the last decade attesting to the association of deoxyribonucleic acid with the surface membranes of certain mammalian cells. In general these findings have generated little interest as it has usually been assumed that this association is a non-specific epiphenomenon. In this study, we show that the isolated cell membranes of human neutrophils possess a DNA binding molecule with a molecular weight of 30,000. The techniques used to demonstrate this DNA binding molecule, namely the use of biotinylated DNA to probe proteins that have been electrophoretically separated and blotted onto nitrocellulose, should find a general applicability in identifying putative cell surface receptors.

Original languageEnglish (US)
Pages (from-to)1034-1039
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number3
DOIs
StatePublished - Aug 16 1984
Externally publishedYes

Fingerprint

Cell membranes
Biotin
Binding Sites
Cell Membrane
DNA
Collodion
DNA Probes
Cell Surface Receptors
Molecules
Neutrophils
Molecular Weight
Membranes
Molecular weight
Cells
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Biotin-labelled DNA : A novel approach for the recognition of a DNA binding site on cell membranes. / Gabor, Gary; Bennett, Robert (Rob).

In: Biochemical and Biophysical Research Communications, Vol. 122, No. 3, 16.08.1984, p. 1034-1039.

Research output: Contribution to journalArticle

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