Binding of epidermal growth factor in rat pancreatic acini

Murray Korc; Lynn M. Matrisian; Stephen R. Planck; Bruce E. Magun

doi:10.1016/0006-291X(83)91408-0

Binding of epidermal growth factor in rat pancreatic acini

Murray Korc, Lynn M. Matrisian, Stephen R. Planck, Bruce E. Magun

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Abstract

Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [³H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.

Original language	English (US)
Pages (from-to)	1066-1073
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	111
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)91408-0
State	Published - Mar 29 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Binding of epidermal growth factor in rat pancreatic acini",

abstract = "Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.",

author = "Murray Korc and Matrisian, {Lynn M.} and Planck, {Stephen R.} and Magun, {Bruce E.}",

note = "Funding Information: by NIH grant",

year = "1983",

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doi = "10.1016/0006-291X(83)91408-0",

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T1 - Binding of epidermal growth factor in rat pancreatic acini

AU - Korc, Murray

AU - Matrisian, Lynn M.

AU - Planck, Stephen R.

AU - Magun, Bruce E.

N1 - Funding Information: by NIH grant

PY - 1983/3/29

Y1 - 1983/3/29

N2 - Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.

AB - Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.

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AN - SCOPUS:0020549315

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EP - 1073

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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Binding of epidermal growth factor in rat pancreatic acini

Abstract

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