Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells

Murray Korc, Bruce E. Magun

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The binding of 125I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture.

Original languageEnglish (US)
Pages (from-to)1849-1855
Number of pages7
JournalLife Sciences
Volume36
Issue number19
DOIs
StatePublished - May 13 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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