Bilayers merge even when exocytosis is transient

Justin W. Taraska, Wolfhard Almers

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

During exocytosis, the lumen of secretory vesicles connects with the extracellular space. In some vesicles, this connection closes again, causing the vesicle to be recaptured mostly intact. The degree to which the bilayers of such vesicles mix with the plasma membrane is unknown. Work supporting the kiss-and-run model of transient exocytosis implies that synaptic vesicles allow neither lipid nor protein to escape into the plasma membrane, suggesting that the two bilayers never merge. Here, we test whether neuroendocrine granules behave similarly. Using two-color evanescent field microscopy, we imaged the lipid probe FM4-64 and fluorescent proteins in single dense core granules. During exocytosis, granules lost FM4-64 into the plasma membrane in small fractions of a second. Although FM4-64 was lost, granules retained the membrane protein, GFP-phogrin. By using GFP-phogrin as a probe for resealing, it was found that even granules that reseal lose FM4-64. We conclude that the lipid bilayers of the granule and the plasma membrane become continuous even when exocytosis is transient.

Original languageEnglish (US)
Pages (from-to)8780-8785
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number23
DOIs
StatePublished - Jun 8 2004

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Exocytosis
Cell Membrane
Lipids
Synaptic Vesicles
Secretory Vesicles
Extracellular Space
Lipid Bilayers
Microscopy
Membrane Proteins
Proteins
Color
FM 4-64

Keywords

  • Evanescent field microscopy
  • FM4-64
  • Kiss-and-run
  • PC12 cells

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Bilayers merge even when exocytosis is transient. / Taraska, Justin W.; Almers, Wolfhard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 23, 08.06.2004, p. 8780-8785.

Research output: Contribution to journalArticle

Taraska, Justin W. ; Almers, Wolfhard. / Bilayers merge even when exocytosis is transient. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 23. pp. 8780-8785.
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