@article{7d37893c724f4a2bbf5eb6c2ab72e25b,
title = "Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain",
abstract = "Pleckstrin homology (PH) domains have been identified only in eukaryotic proteins to date. We have determined crystal structures for three members of an uncharacterized protein family (Pfam PF08000), which provide compelling evidence for the existence of PH-like domains in bacteria (PHb). The first two structures contain a single PHb domain that forms a dome-shaped, oligomeric ring with C5 symmetry. The third structure has an additional helical hairpin attached at the C-terminus and forms a similar but much larger ring with C12 symmetry. Thus, both molecular assemblies exhibit rare, higher-order, cyclic symmetry but preserve a similar arrangement of their PHb domains, which gives rise to a conserved hydrophilic surface at the intersection of the β-strands of adjacent protomers that likely mediates protein-protein interactions. As a result of these structures, additional families of PHb domains were identified, suggesting that PH domains are much more widespread than originally anticipated. Thus, rather than being a eukaryotic innovation, the PH domain superfamily appears to have existed before prokaryotes and eukaryotes diverged.",
keywords = "Pleckstrin homology (PH) domain, bacterial PH domain (PHb), higher-order symmetry, protein assembly, protein evolution",
author = "Qingping Xu and Alex Bateman and Finn, {Robert D.} and Polat Abdubek and Tamara Astakhova and Axelrod, {Herbert L.} and Constantina Bakolitsa and Dennis Carlton and Connie Chen and Chiu, {Hsiu Ju} and Michelle Chiu and Thomas Clayton and Debanu Das and Deller, {Marc C.} and Lian Duan and Kyle Ellrott and Dustin Ernst and Farr, {Carol L.} and Julie Feuerhelm and Grant, {Joanna C.} and Anna Grzechnik and Han, {Gye Won} and Lukasz Jaroszewski and Jin, {Kevin K.} and Klock, {Heath E.} and Knuth, {Mark W.} and Piotr Kozbial and Krishna, {S. Sri} and Abhinav Kumar and David Marciano and Daniel McMullan and Miller, {Mitchell D.} and Morse, {Andrew T.} and Edward Nigoghossian and Amanda Nopakun and Linda Okach and Christina Puckett and Ron Reyes and Rife, {Christopher L.} and Natasha Sefcovic and Tien, {Henry J.} and Trame, {Christine B.} and {van den Bedem}, Henry and Dana Weekes and Tiffany Wooten and Hodgson, {Keith O.} and John Wooley and Elsliger, {Marc Andr{\'e}} and Deacon, {Ashley M.} and Adam Godzik and Lesley, {Scott A.} and Wilson, {Ian A.}",
note = "Funding Information: The project is sponsored by National Institute of General Medical Sciences Protein Structure Initiative (grant number U54 GM074898 to the JCSG). A.B. and R.D.F. are supported by the Wellcome Trust (grant number WT077044/Z/05/Z ). Portions of this research were carried out at the SSRL and the ALS. The SSRL is a national user facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences. The SSRL Structural Molecular Biology Program is supported by the DOE , Office of Biological and Environmental Research , and by the National Institutes of Health . The ALS is supported by the Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division, of the U.S . DOE under Contract No. DE-AC03-76SF00098 at Lawrence Berkeley National Laboratory. Exiguobacterium sp. 255-15 was a gift from Drs. Tamara Cole and Jim Tiedje, Michigan State University, East Lansing, MI, USA. Shewanella sp. PV-4 and S. amazonensis SB2B were gifts from Dr. Jim Fredrickson, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. The content of this work is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences. We thank Dr. Todd O. Yeates for insightful comments. ",
year = "2010",
month = feb,
day = "12",
doi = "10.1016/j.jmb.2009.11.006",
language = "English (US)",
volume = "396",
pages = "31--46",
journal = "Journal of molecular biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",
}