B lymphocyte development and activation independent of MHC class II expression

J. S. Markowitz, P. R. Rogers, M. J. Grusby, D. C. Parker, L. H. Glimcher

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54 Scopus citations

Abstract

A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgG1 (and, variably, IgE) compared to control. The antibody response against thymic- independent Ag was intact in class II animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell- independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.

Original languageEnglish (US)
Pages (from-to)1223-1233
Number of pages11
JournalJournal of Immunology
Volume150
Issue number4
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Markowitz, J. S., Rogers, P. R., Grusby, M. J., Parker, D. C., & Glimcher, L. H. (1993). B lymphocyte development and activation independent of MHC class II expression. Journal of Immunology, 150(4), 1223-1233.