TY - JOUR
T1 - B lymphocyte development and activation independent of MHC class II expression
AU - Markowitz, Jay S.
AU - Rogers, Paul R.
AU - Grusby, Michael J.
AU - Parker, David C.
AU - Glimcher, Laurie H.
PY - 1993
Y1 - 1993
N2 - A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.
AB - A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.
UR - http://www.scopus.com/inward/record.url?scp=0027156770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027156770&partnerID=8YFLogxK
M3 - Article
C2 - 8432977
AN - SCOPUS:0027156770
SN - 0022-1767
VL - 150
SP - 1223
EP - 1233
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -