B lymphocyte development and activation independent of MHC class II expression

Jay S. Markowitz, Paul R. Rogers, Michael J. Grusby, David Parker, Laurie H. Glimcher

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.

Original languageEnglish (US)
Pages (from-to)1223-1233
Number of pages11
JournalJournal of Immunology
Volume150
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Lymphocyte Activation
B-Lymphocytes
T-Lymphocytes
Immunoglobulin M
Immunoglobulin D
B-Lymphoid Precursor Cells
Immunoglobulin E
Antibody Formation
Bone Marrow
Serum
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Markowitz, J. S., Rogers, P. R., Grusby, M. J., Parker, D., & Glimcher, L. H. (1993). B lymphocyte development and activation independent of MHC class II expression. Journal of Immunology, 150(4), 1223-1233.

B lymphocyte development and activation independent of MHC class II expression. / Markowitz, Jay S.; Rogers, Paul R.; Grusby, Michael J.; Parker, David; Glimcher, Laurie H.

In: Journal of Immunology, Vol. 150, No. 4, 1993, p. 1223-1233.

Research output: Contribution to journalArticle

Markowitz, JS, Rogers, PR, Grusby, MJ, Parker, D & Glimcher, LH 1993, 'B lymphocyte development and activation independent of MHC class II expression', Journal of Immunology, vol. 150, no. 4, pp. 1223-1233.
Markowitz JS, Rogers PR, Grusby MJ, Parker D, Glimcher LH. B lymphocyte development and activation independent of MHC class II expression. Journal of Immunology. 1993;150(4):1223-1233.
Markowitz, Jay S. ; Rogers, Paul R. ; Grusby, Michael J. ; Parker, David ; Glimcher, Laurie H. / B lymphocyte development and activation independent of MHC class II expression. In: Journal of Immunology. 1993 ; Vol. 150, No. 4. pp. 1223-1233.
@article{9143295792834611b4000b22588dc15b,
title = "B lymphocyte development and activation independent of MHC class II expression",
abstract = "A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.",
author = "Markowitz, {Jay S.} and Rogers, {Paul R.} and Grusby, {Michael J.} and David Parker and Glimcher, {Laurie H.}",
year = "1993",
language = "English (US)",
volume = "150",
pages = "1223--1233",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - B lymphocyte development and activation independent of MHC class II expression

AU - Markowitz, Jay S.

AU - Rogers, Paul R.

AU - Grusby, Michael J.

AU - Parker, David

AU - Glimcher, Laurie H.

PY - 1993

Y1 - 1993

N2 - A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.

AB - A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgGI1(and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II- animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.

UR - http://www.scopus.com/inward/record.url?scp=0027156770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027156770&partnerID=8YFLogxK

M3 - Article

C2 - 8432977

AN - SCOPUS:0027156770

VL - 150

SP - 1223

EP - 1233

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -