Autonomic and enteric neurons: Cell culture

P. J. Lein; A. D. Fryer

doi:10.1016/B978-0-12-809324-5.23365-8

Autonomic and enteric neurons: Cell culture

P. J. Lein, A. D. Fryer

Pulmonary & Critical Care Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Sympathetic cell culture is widely used for mechanistic studies of neurodevelopment, plasticity and neurotransmission. Other than chick ciliary ganglia, parasympathetic and enteric cultures are not as widely used, and have largely focused on electrophysiological and pharmacological properties of these cells. New procedures for culturing primary human autonomic neurons, and for co-culturing autonomic neurons with target tissue cells will increase the power of these models for addressing significant gaps in understanding of the cell biology and pharmacology of these neurons, the impact of which is underscored by the importance of autonomic nervous systems in regulating all major organ systems in vertebrates.

Original language	English (US)
Title of host publication	The Curated Reference Collection in Neuroscience and Biobehavioral Psychology
Publisher	Elsevier Science Ltd.
ISBN (Electronic)	9780128093245
DOIs	https://doi.org/10.1016/B978-0-12-809324-5.23365-8
State	Published - Jan 1 2016

Keywords

Campenot chamber
Ciliary ganglia
Enteric neurons
Ganglionic glia
Human peripheral neurons
In vitro models
Microelectrode arrays
Myenteric ganglia
Neural crest cells
Neurotrophic factors
Parasympathetic neurons
Rose chamber
Superior cervical ganglia
Sympathetic neurons
Tissue culture

ASJC Scopus subject areas

Medicine(all)

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10.1016/B978-0-12-809324-5.23365-8

Cite this

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abstract = "Sympathetic cell culture is widely used for mechanistic studies of neurodevelopment, plasticity and neurotransmission. Other than chick ciliary ganglia, parasympathetic and enteric cultures are not as widely used, and have largely focused on electrophysiological and pharmacological properties of these cells. New procedures for culturing primary human autonomic neurons, and for co-culturing autonomic neurons with target tissue cells will increase the power of these models for addressing significant gaps in understanding of the cell biology and pharmacology of these neurons, the impact of which is underscored by the importance of autonomic nervous systems in regulating all major organ systems in vertebrates.",

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KW - Ganglionic glia

KW - Human peripheral neurons

KW - In vitro models

KW - Microelectrode arrays

KW - Myenteric ganglia

KW - Neural crest cells

KW - Neurotrophic factors

KW - Parasympathetic neurons

KW - Rose chamber

KW - Superior cervical ganglia

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KW - Tissue culture

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Autonomic and enteric neurons: Cell culture

Abstract

Keywords

ASJC Scopus subject areas

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