Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring

Adetunji P. Fayomi, Karen Peters, Meena Sukhwani, Hanna Valli-Pulaski, Gunapala Shetty, Marvin L. Meistrich, Lisa Houser, Nicola Robertson, Victoria Roberts, Cathy Ramsey, Carol Hanna, Jon Hennebold, Ina Dobrinski, Kyle E. Orwig

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.

    Original languageEnglish (US)
    Pages (from-to)1314-1319
    Number of pages6
    JournalScience
    Volume363
    Issue number6433
    DOIs
    StatePublished - Mar 22 2019

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    Spermatozoa
    Testis
    Transplants
    Tissue Transplantation
    Skin
    Cryopreservation
    Spermatogenesis
    Macaca mulatta
    Oocytes
    Embryonic Development
    Fertility
    Testosterone
    Observation
    Parturition
    Pregnancy
    Neoplasms
    Therapeutics

    ASJC Scopus subject areas

    • General

    Cite this

    Fayomi, A. P., Peters, K., Sukhwani, M., Valli-Pulaski, H., Shetty, G., Meistrich, M. L., ... Orwig, K. E. (2019). Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. Science, 363(6433), 1314-1319. https://doi.org/10.1126/science.aav2914

    Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. / Fayomi, Adetunji P.; Peters, Karen; Sukhwani, Meena; Valli-Pulaski, Hanna; Shetty, Gunapala; Meistrich, Marvin L.; Houser, Lisa; Robertson, Nicola; Roberts, Victoria; Ramsey, Cathy; Hanna, Carol; Hennebold, Jon; Dobrinski, Ina; Orwig, Kyle E.

    In: Science, Vol. 363, No. 6433, 22.03.2019, p. 1314-1319.

    Research output: Contribution to journalArticle

    Fayomi, AP, Peters, K, Sukhwani, M, Valli-Pulaski, H, Shetty, G, Meistrich, ML, Houser, L, Robertson, N, Roberts, V, Ramsey, C, Hanna, C, Hennebold, J, Dobrinski, I & Orwig, KE 2019, 'Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring', Science, vol. 363, no. 6433, pp. 1314-1319. https://doi.org/10.1126/science.aav2914
    Fayomi AP, Peters K, Sukhwani M, Valli-Pulaski H, Shetty G, Meistrich ML et al. Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. Science. 2019 Mar 22;363(6433):1314-1319. https://doi.org/10.1126/science.aav2914
    Fayomi, Adetunji P. ; Peters, Karen ; Sukhwani, Meena ; Valli-Pulaski, Hanna ; Shetty, Gunapala ; Meistrich, Marvin L. ; Houser, Lisa ; Robertson, Nicola ; Roberts, Victoria ; Ramsey, Cathy ; Hanna, Carol ; Hennebold, Jon ; Dobrinski, Ina ; Orwig, Kyle E. / Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. In: Science. 2019 ; Vol. 363, No. 6433. pp. 1314-1319.
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