Abstract
Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1314-1319 |
| Number of pages | 6 |
| Journal | Science |
| Volume | 363 |
| Issue number | 6433 |
| DOIs | |
| State | Published - Mar 22 2019 |
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Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. / Fayomi, Adetunji P.; Peters, Karen; Sukhwani, Meena; Valli-Pulaski, Hanna; Shetty, Gunapala; Meistrich, Marvin L.; Houser, Lisa; Robertson, Nicola; Roberts, Victoria; Ramsey, Cathy; Hanna, Carol; Hennebold, Jon; Dobrinski, Ina; Orwig, Kyle E.
In: Science, Vol. 363, No. 6433, 22.03.2019, p. 1314-1319.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring
AU - Fayomi, Adetunji P.
AU - Peters, Karen
AU - Sukhwani, Meena
AU - Valli-Pulaski, Hanna
AU - Shetty, Gunapala
AU - Meistrich, Marvin L.
AU - Houser, Lisa
AU - Robertson, Nicola
AU - Roberts, Victoria
AU - Ramsey, Cathy
AU - Hanna, Carol
AU - Hennebold, Jon
AU - Dobrinski, Ina
AU - Orwig, Kyle E.
PY - 2019/3/22
Y1 - 2019/3/22
N2 - Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.
AB - Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.
UR - http://www.scopus.com/inward/record.url?scp=85063661598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063661598&partnerID=8YFLogxK
U2 - 10.1126/science.aav2914
DO - 10.1126/science.aav2914
M3 - Article
C2 - 30898927
AN - SCOPUS:85063661598
VL - 363
SP - 1314
EP - 1319
JO - Science
JF - Science
SN - 0036-8075
IS - 6433
ER -