@article{59882a4edcf44b759ea239301e7c27e0,
title = "Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring",
abstract = "Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.",
author = "Fayomi, {Adetunji P.} and Karen Peters and Meena Sukhwani and Hanna Valli-Pulaski and Gunapala Shetty and Meistrich, {Marvin L.} and Lisa Houser and Nicola Robertson and Victoria Roberts and Cathy Ramsey and Carol Hanna and Hennebold, {Jon D.} and Ina Dobrinski and Orwig, {Kyle E.}",
note = "Funding Information: We thank the Lab Animal Research Resource (LARR) staff of Magee-Womens Research Institute for animal husbandry and technical support. Histology was performed by the Histology Core of Magee-Womens Research Institute. FSH assays were performed by the Endocrine Technologies Support Core at the Oregon National Primate Research Center. This work was supported by NICHD grants P01 HD075795 and R01 HD076412 to K.E.O.; a diversity supplement to HD076412 for A.P.F.; NIH P51 OD011092 to the Oregon National Primate Research Center (J.D.H.); and the Magee-Womens Research Institute and Foundation.",
year = "2019",
month = mar,
day = "22",
doi = "10.1126/science.aav2914",
language = "English (US)",
volume = "363",
pages = "1314--1319",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6433",
}