Abstract
Oxidative modification of the major cholesterol carrying lipoprotein, low-density lipoprotein (LDL), renders it more atherogenic as well as inducing unique fluorescence spectral characteristics that distinguish it from native (non-oxidized) LDL. This fluorescence feature has been identified using a microspectrofluorometry system capable of recording autofluorescence of individual cultured macrophages incubated with oxidized LDL. Differences in fluorescence spectra between individual control cells and oxidized LDL loaded cells could also be elicited using dye-enhanced fluorescence with neutral lipid probes such as nile red. Autofluorescence spectroscopy applied to the detection of intracellular oxidized LDL accumulation in circulating monocytes may be useful for identifying a novel risk factor in the assessment of atherosclerosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 2679 |
| DOIs | |
| State | Published - 1996 |
| Externally published | Yes |
| Event | Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy - San Jose, CA, United States Duration: Jan 29 1996 → Jan 29 1996 |
Keywords
- Atherosclerosis
- Autofluorescence
- Dye-enhanced fluorescence
- Microspectrofluorometry
- Monocyte/macrophage
- Oxidized low density lipoprotein
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering