Autofluorescence detection of oxidized LDL in monocytes

A novel risk factor for the assessment of atherosclerosis?

Tami N. Fink, Alexander A. Oraevsky, Frank K. Tittel, Sharon L. Thomsen, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Oxidative modification of the major cholesterol carrying lipoprotein, low-density lipoprotein (LDL), renders it more atherogenic as well as inducing unique fluorescence spectral characteristics that distinguish it from native (non-oxidized) LDL. This fluorescence feature has been identified using a microspectrofluorometry system capable of recording autofluorescence of individual cultured macrophages incubated with oxidized LDL. Differences in fluorescence spectra between individual control cells and oxidized LDL loaded cells could also be elicited using dye-enhanced fluorescence with neutral lipid probes such as nile red. Autofluorescence spectroscopy applied to the detection of intracellular oxidized LDL accumulation in circulating monocytes may be useful for identifying a novel risk factor in the assessment of atherosclerosis.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages34-39
Number of pages6
Volume2679
DOIs
StatePublished - 1996
Externally publishedYes
EventAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy - San Jose, CA, United States
Duration: Jan 29 1996Jan 29 1996

Other

OtherAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
CountryUnited States
CitySan Jose, CA
Period1/29/961/29/96

Fingerprint

Autofluorescence
monocytes
Monocytes
lipoproteins
Atherosclerosis
arteriosclerosis
Lipoproteins
Risk Factors
Fluorescence
LDL Lipoproteins
fluorescence
Macrophages
Macrophage
macrophages
Coloring Agents
Cholesterol
cholesterol
Cell
Spectroscopy
Lipids

Keywords

  • Atherosclerosis
  • Autofluorescence
  • Dye-enhanced fluorescence
  • Microspectrofluorometry
  • Monocyte/macrophage
  • Oxidized low density lipoprotein

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Fink, T. N., Oraevsky, A. A., Tittel, F. K., Thomsen, S. L., & Jacques, S. (1996). Autofluorescence detection of oxidized LDL in monocytes: A novel risk factor for the assessment of atherosclerosis? In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2679, pp. 34-39) https://doi.org/10.1117/12.237595

Autofluorescence detection of oxidized LDL in monocytes : A novel risk factor for the assessment of atherosclerosis? / Fink, Tami N.; Oraevsky, Alexander A.; Tittel, Frank K.; Thomsen, Sharon L.; Jacques, Steven.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679 1996. p. 34-39.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fink, TN, Oraevsky, AA, Tittel, FK, Thomsen, SL & Jacques, S 1996, Autofluorescence detection of oxidized LDL in monocytes: A novel risk factor for the assessment of atherosclerosis? in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2679, pp. 34-39, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, San Jose, CA, United States, 1/29/96. https://doi.org/10.1117/12.237595
Fink TN, Oraevsky AA, Tittel FK, Thomsen SL, Jacques S. Autofluorescence detection of oxidized LDL in monocytes: A novel risk factor for the assessment of atherosclerosis? In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679. 1996. p. 34-39 https://doi.org/10.1117/12.237595
Fink, Tami N. ; Oraevsky, Alexander A. ; Tittel, Frank K. ; Thomsen, Sharon L. ; Jacques, Steven. / Autofluorescence detection of oxidized LDL in monocytes : A novel risk factor for the assessment of atherosclerosis?. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679 1996. pp. 34-39
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abstract = "Oxidative modification of the major cholesterol carrying lipoprotein, low-density lipoprotein (LDL), renders it more atherogenic as well as inducing unique fluorescence spectral characteristics that distinguish it from native (non-oxidized) LDL. This fluorescence feature has been identified using a microspectrofluorometry system capable of recording autofluorescence of individual cultured macrophages incubated with oxidized LDL. Differences in fluorescence spectra between individual control cells and oxidized LDL loaded cells could also be elicited using dye-enhanced fluorescence with neutral lipid probes such as nile red. Autofluorescence spectroscopy applied to the detection of intracellular oxidized LDL accumulation in circulating monocytes may be useful for identifying a novel risk factor in the assessment of atherosclerosis.",
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