Augmented pro-apoptotic effects of TRAIL and proteasome inhibitor in human promonocytic leukemic U937 cells

I. Mlynarczuk; G. Hoser; T. Grzela; T. Stoklosa; C. Wójcik; J. Malejczyk; M. Jakóbisiak

Augmented pro-apoptotic effects of TRAIL and proteasome inhibitor in human promonocytic leukemic U937 cells

I. Mlynarczuk, G. Hoser, T. Grzela, T. Stoklosa, C. Wójcik, J. Malejczyk, M. Jakóbisiak

Family Medicine

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8 Scopus citations

Abstract

TRAIL, Tumor necrosis factor-related apoptosis-inducing ligand, a member of the TNF family, is known to be cytotoxic for a high proportion of rumor cell lines. However, successful application of TRAIL in rumor therapy may depend on finding other agents that can potentiate its antitumor effects. The present study showed that the cytostatic/cytotoxic TRAIL activity against U937 cells could be significantly augmented by proteasome inhibitor PSI, as revealed by MTT assay. Increased cytostatic/cytotoxic effect on U937 cells by TRAIL/PSI combined treatment was caused by apoptosis, as shown by an increased PARP cleavage rate. TRAIL/PSI did not affect the level of mRNA expression for TRAIL receptors (DR4, DR5, DcR1) and other apoptosis signal transduction molecules (TRADD, caspase-8).

Original language	English (US)
Pages (from-to)	1237-1240
Number of pages	4
Journal	Anticancer research
Volume	21
Issue number	2 A
State	Published - Jun 11 2001

Keywords

Apoptosis
Proteasome inhibitor
TRAIL
U937 cells

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Augmented pro-apoptotic effects of TRAIL and proteasome inhibitor in human promonocytic leukemic U937 cells",

abstract = "TRAIL, Tumor necrosis factor-related apoptosis-inducing ligand, a member of the TNF family, is known to be cytotoxic for a high proportion of rumor cell lines. However, successful application of TRAIL in rumor therapy may depend on finding other agents that can potentiate its antitumor effects. The present study showed that the cytostatic/cytotoxic TRAIL activity against U937 cells could be significantly augmented by proteasome inhibitor PSI, as revealed by MTT assay. Increased cytostatic/cytotoxic effect on U937 cells by TRAIL/PSI combined treatment was caused by apoptosis, as shown by an increased PARP cleavage rate. TRAIL/PSI did not affect the level of mRNA expression for TRAIL receptors (DR4, DR5, DcR1) and other apoptosis signal transduction molecules (TRADD, caspase-8).",

keywords = "Apoptosis, Proteasome inhibitor, TRAIL, U937 cells",

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TY - JOUR

T1 - Augmented pro-apoptotic effects of TRAIL and proteasome inhibitor in human promonocytic leukemic U937 cells

AU - Mlynarczuk, I.

AU - Hoser, G.

AU - Grzela, T.

AU - Stoklosa, T.

AU - Wójcik, C.

AU - Malejczyk, J.

AU - Jakóbisiak, M.

PY - 2001/6/11

Y1 - 2001/6/11

N2 - TRAIL, Tumor necrosis factor-related apoptosis-inducing ligand, a member of the TNF family, is known to be cytotoxic for a high proportion of rumor cell lines. However, successful application of TRAIL in rumor therapy may depend on finding other agents that can potentiate its antitumor effects. The present study showed that the cytostatic/cytotoxic TRAIL activity against U937 cells could be significantly augmented by proteasome inhibitor PSI, as revealed by MTT assay. Increased cytostatic/cytotoxic effect on U937 cells by TRAIL/PSI combined treatment was caused by apoptosis, as shown by an increased PARP cleavage rate. TRAIL/PSI did not affect the level of mRNA expression for TRAIL receptors (DR4, DR5, DcR1) and other apoptosis signal transduction molecules (TRADD, caspase-8).

AB - TRAIL, Tumor necrosis factor-related apoptosis-inducing ligand, a member of the TNF family, is known to be cytotoxic for a high proportion of rumor cell lines. However, successful application of TRAIL in rumor therapy may depend on finding other agents that can potentiate its antitumor effects. The present study showed that the cytostatic/cytotoxic TRAIL activity against U937 cells could be significantly augmented by proteasome inhibitor PSI, as revealed by MTT assay. Increased cytostatic/cytotoxic effect on U937 cells by TRAIL/PSI combined treatment was caused by apoptosis, as shown by an increased PARP cleavage rate. TRAIL/PSI did not affect the level of mRNA expression for TRAIL receptors (DR4, DR5, DcR1) and other apoptosis signal transduction molecules (TRADD, caspase-8).

KW - Apoptosis

KW - Proteasome inhibitor

KW - TRAIL

KW - U937 cells

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M3 - Article

C2 - 11396170

AN - SCOPUS:0035015142

SN - 0250-7005

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JO - Anticancer Research

JF - Anticancer Research

IS - 2 A

ER -

Augmented pro-apoptotic effects of TRAIL and proteasome inhibitor in human promonocytic leukemic U937 cells

Abstract

Keywords

ASJC Scopus subject areas

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