Atopic dermatitis in adults

Evaluation of peripheral blood mononuclear cells proliferation response to Staphylococcus aureus enterotoxins A and B and analysis of interleukin-18 secretion

Raquel Leão Orfali, Maria Notomi Sato, Roberto Takaoka, Mayce Helena Azor, Evandro Ararigbóia Rivitti, Jon Hanifin, Valéria Aoki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Atopic dermatitis (AD) is a chronic, inflammatory skin disease with a high prevalence and complex pathogenesis. The skin of AD patients is usually colonized by Staphylococcus aureus (S. aureus); its exotoxins may trigger or enhance the cutaneous inflammation. Several mediators are related to the AD immune imbalance and interleukin-18 (IL-18), an inflammatory cytokine, may play a role in the atopic skin inflammation. Aims: To evaluate peripheral blood mononuclear cells (PBMC) proliferation response to staphylococcal enterotoxins A (SEA) and B (SEB) and the levels of IL-18 in adults with AD. Methods: Thirty-eight adult patients with AD and 33 healthy controls were analysed. PBMC were stimulated with SEA and SEB, phytohemaglutinin (PHA), pokeweed (PWM), tetanus toxoid (TT) and Candida albicans (CMA). IL-18 secretion from PBMC culture supernatants and sera were measured by ELISA. Results: A significant inhibition of the PBMC proliferation response to SEA, PHA, TT and CMA of AD patients was detected (P ≤ 0.05). Furthermore, increased levels of IL-18 were detected both in sera and non-stimulated PBMC culture supernatants from AD patients (P ≤ 0.05). Conclusions: A decreased PBMC proliferation response to distinct antigens and mitogens (TT, CMA, SEA and PHA) in adults with AD suggest a compromised immune profile. IL-18 secretion from AD upon stimulation was similar from controls, which may indicate a diverse mechanism of skin inflammation maintained by Staphylococcus aureus. On the other hand, augmented IL-18 secretion from AD sera and non-stimulated cell culture may enhance the immune dysfunction observed in AD, leading to constant skin inflammation.

Original languageEnglish (US)
Pages (from-to)628-633
Number of pages6
JournalExperimental Dermatology
Volume18
Issue number7
DOIs
StatePublished - 2009

Fingerprint

Interleukin-18
Enterotoxins
Cell proliferation
Atopic Dermatitis
Staphylococcus aureus
Blood Cells
Blood
Cell Proliferation
Skin
Tetanus Toxoid
Cell culture
Inflammation
Cell Culture Techniques
Phytolacca americana
Serum
Exotoxins
Candida
Candida albicans
Mitogens
Skin Diseases

Keywords

  • Atopic dermatitis
  • Interleukin 18
  • Proliferation response
  • Staphylococcus enterotoxins

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

Atopic dermatitis in adults : Evaluation of peripheral blood mononuclear cells proliferation response to Staphylococcus aureus enterotoxins A and B and analysis of interleukin-18 secretion. / Orfali, Raquel Leão; Sato, Maria Notomi; Takaoka, Roberto; Azor, Mayce Helena; Rivitti, Evandro Ararigbóia; Hanifin, Jon; Aoki, Valéria.

In: Experimental Dermatology, Vol. 18, No. 7, 2009, p. 628-633.

Research output: Contribution to journalArticle

Orfali, Raquel Leão ; Sato, Maria Notomi ; Takaoka, Roberto ; Azor, Mayce Helena ; Rivitti, Evandro Ararigbóia ; Hanifin, Jon ; Aoki, Valéria. / Atopic dermatitis in adults : Evaluation of peripheral blood mononuclear cells proliferation response to Staphylococcus aureus enterotoxins A and B and analysis of interleukin-18 secretion. In: Experimental Dermatology. 2009 ; Vol. 18, No. 7. pp. 628-633.
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abstract = "Background: Atopic dermatitis (AD) is a chronic, inflammatory skin disease with a high prevalence and complex pathogenesis. The skin of AD patients is usually colonized by Staphylococcus aureus (S. aureus); its exotoxins may trigger or enhance the cutaneous inflammation. Several mediators are related to the AD immune imbalance and interleukin-18 (IL-18), an inflammatory cytokine, may play a role in the atopic skin inflammation. Aims: To evaluate peripheral blood mononuclear cells (PBMC) proliferation response to staphylococcal enterotoxins A (SEA) and B (SEB) and the levels of IL-18 in adults with AD. Methods: Thirty-eight adult patients with AD and 33 healthy controls were analysed. PBMC were stimulated with SEA and SEB, phytohemaglutinin (PHA), pokeweed (PWM), tetanus toxoid (TT) and Candida albicans (CMA). IL-18 secretion from PBMC culture supernatants and sera were measured by ELISA. Results: A significant inhibition of the PBMC proliferation response to SEA, PHA, TT and CMA of AD patients was detected (P ≤ 0.05). Furthermore, increased levels of IL-18 were detected both in sera and non-stimulated PBMC culture supernatants from AD patients (P ≤ 0.05). Conclusions: A decreased PBMC proliferation response to distinct antigens and mitogens (TT, CMA, SEA and PHA) in adults with AD suggest a compromised immune profile. IL-18 secretion from AD upon stimulation was similar from controls, which may indicate a diverse mechanism of skin inflammation maintained by Staphylococcus aureus. On the other hand, augmented IL-18 secretion from AD sera and non-stimulated cell culture may enhance the immune dysfunction observed in AD, leading to constant skin inflammation.",
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T1 - Atopic dermatitis in adults

T2 - Evaluation of peripheral blood mononuclear cells proliferation response to Staphylococcus aureus enterotoxins A and B and analysis of interleukin-18 secretion

AU - Orfali, Raquel Leão

AU - Sato, Maria Notomi

AU - Takaoka, Roberto

AU - Azor, Mayce Helena

AU - Rivitti, Evandro Ararigbóia

AU - Hanifin, Jon

AU - Aoki, Valéria

PY - 2009

Y1 - 2009

N2 - Background: Atopic dermatitis (AD) is a chronic, inflammatory skin disease with a high prevalence and complex pathogenesis. The skin of AD patients is usually colonized by Staphylococcus aureus (S. aureus); its exotoxins may trigger or enhance the cutaneous inflammation. Several mediators are related to the AD immune imbalance and interleukin-18 (IL-18), an inflammatory cytokine, may play a role in the atopic skin inflammation. Aims: To evaluate peripheral blood mononuclear cells (PBMC) proliferation response to staphylococcal enterotoxins A (SEA) and B (SEB) and the levels of IL-18 in adults with AD. Methods: Thirty-eight adult patients with AD and 33 healthy controls were analysed. PBMC were stimulated with SEA and SEB, phytohemaglutinin (PHA), pokeweed (PWM), tetanus toxoid (TT) and Candida albicans (CMA). IL-18 secretion from PBMC culture supernatants and sera were measured by ELISA. Results: A significant inhibition of the PBMC proliferation response to SEA, PHA, TT and CMA of AD patients was detected (P ≤ 0.05). Furthermore, increased levels of IL-18 were detected both in sera and non-stimulated PBMC culture supernatants from AD patients (P ≤ 0.05). Conclusions: A decreased PBMC proliferation response to distinct antigens and mitogens (TT, CMA, SEA and PHA) in adults with AD suggest a compromised immune profile. IL-18 secretion from AD upon stimulation was similar from controls, which may indicate a diverse mechanism of skin inflammation maintained by Staphylococcus aureus. On the other hand, augmented IL-18 secretion from AD sera and non-stimulated cell culture may enhance the immune dysfunction observed in AD, leading to constant skin inflammation.

AB - Background: Atopic dermatitis (AD) is a chronic, inflammatory skin disease with a high prevalence and complex pathogenesis. The skin of AD patients is usually colonized by Staphylococcus aureus (S. aureus); its exotoxins may trigger or enhance the cutaneous inflammation. Several mediators are related to the AD immune imbalance and interleukin-18 (IL-18), an inflammatory cytokine, may play a role in the atopic skin inflammation. Aims: To evaluate peripheral blood mononuclear cells (PBMC) proliferation response to staphylococcal enterotoxins A (SEA) and B (SEB) and the levels of IL-18 in adults with AD. Methods: Thirty-eight adult patients with AD and 33 healthy controls were analysed. PBMC were stimulated with SEA and SEB, phytohemaglutinin (PHA), pokeweed (PWM), tetanus toxoid (TT) and Candida albicans (CMA). IL-18 secretion from PBMC culture supernatants and sera were measured by ELISA. Results: A significant inhibition of the PBMC proliferation response to SEA, PHA, TT and CMA of AD patients was detected (P ≤ 0.05). Furthermore, increased levels of IL-18 were detected both in sera and non-stimulated PBMC culture supernatants from AD patients (P ≤ 0.05). Conclusions: A decreased PBMC proliferation response to distinct antigens and mitogens (TT, CMA, SEA and PHA) in adults with AD suggest a compromised immune profile. IL-18 secretion from AD upon stimulation was similar from controls, which may indicate a diverse mechanism of skin inflammation maintained by Staphylococcus aureus. On the other hand, augmented IL-18 secretion from AD sera and non-stimulated cell culture may enhance the immune dysfunction observed in AD, leading to constant skin inflammation.

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KW - Interleukin 18

KW - Proliferation response

KW - Staphylococcus enterotoxins

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