TY - JOUR
T1 - Association between the PDGF receptor and members of the src family of tyrosine kinases
AU - Kypta, Robert M.
AU - Goldberg, Yves
AU - Ulug, Emin T.
AU - Courtneidge, Sara A.
N1 - Funding Information:
We thank Calle Heldin and his colleagues for their generosity with reagents and advice, Seng Cheng and Rodrigo Bravo for gifts of cell lines, Heinrich Gausepohl and Rainer Frank for peptide synthesis, Joan Brugge and Sally Parsons for monoclonal antibodies, Giulio Draetta for advice on Western blotting, and Angel&a Heber and Andy Cartwright for technical assistance. Y. G. was supported by an EMBO fellowship, and E. T. U. was supported by Boehringer Ingelheim.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1990/8/10
Y1 - 1990/8/10
N2 - We have examined the interaction between the plate-let-derived growth factor (PDGF) receptor and three src family tyrosine kinases, pp60c-src, p59fyn, and pp62c-yes. The kinase activities of all three enzymes were elevated after PDGF stimulation of quiescent fibroblasts, coincident with association of the src family kinases with the PDGF receptor and other proteins. The presence of a protein of 81-85 kd in these complexes correlated with the detection of phosphatidylinositol (PI) kinase activity (previously described to associate with both the PDGF receptor and pp60c-src-middle T antigen). These results suggest that the physiological response to PDGF involves interaction of the receptor not only with serine/threonine and lipid kinases and a phospholipase, but also with other tyrosine kinases.
AB - We have examined the interaction between the plate-let-derived growth factor (PDGF) receptor and three src family tyrosine kinases, pp60c-src, p59fyn, and pp62c-yes. The kinase activities of all three enzymes were elevated after PDGF stimulation of quiescent fibroblasts, coincident with association of the src family kinases with the PDGF receptor and other proteins. The presence of a protein of 81-85 kd in these complexes correlated with the detection of phosphatidylinositol (PI) kinase activity (previously described to associate with both the PDGF receptor and pp60c-src-middle T antigen). These results suggest that the physiological response to PDGF involves interaction of the receptor not only with serine/threonine and lipid kinases and a phospholipase, but also with other tyrosine kinases.
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U2 - 10.1016/0092-8674(90)90013-5
DO - 10.1016/0092-8674(90)90013-5
M3 - Article
C2 - 1696179
AN - SCOPUS:0025172811
VL - 62
SP - 481
EP - 492
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -