Assessing Genetic Expression Profiles in Melanoma Diagnosis

Sancy Leachman, Stephanie Mengden Koon, Veselina Korcheva, Kevin White

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Most melanocytic tumors can be characterized as a benign nevus or a melanoma by a trained pathologist using traditional histopathological methods. However, a minority demonstrates ambiguous features and continues to be a diagnostic challenge. Genetic expression profiling (GEP) assays have been developed in an effort to resolve this dilemma. These assays measure mRNA levels of specified genes using reverse transcription quantitative polymerase chain reaction technology. The development of GEP assays, methodology, challenges associated with GEP validation and testing, and the suitability of a currently available GEP test for clinical use are reviewed.

Original languageEnglish (US)
JournalDermatologic Clinics
DOIs
StateAccepted/In press - 2017

Fingerprint

Nevus
Reverse Transcription
Melanoma
Technology
Polymerase Chain Reaction
Messenger RNA
Genes
Neoplasms
Pathologists

Keywords

  • Diagnostic test
  • Genetic expression profiling
  • GEP
  • Melanoma
  • RT-PCR

ASJC Scopus subject areas

  • Dermatology

Cite this

Assessing Genetic Expression Profiles in Melanoma Diagnosis. / Leachman, Sancy; Mengden Koon, Stephanie; Korcheva, Veselina; White, Kevin.

In: Dermatologic Clinics, 2017.

Research output: Contribution to journalArticle

@article{23240b9b7c8e44888802ae732e8d8965,
title = "Assessing Genetic Expression Profiles in Melanoma Diagnosis",
abstract = "Most melanocytic tumors can be characterized as a benign nevus or a melanoma by a trained pathologist using traditional histopathological methods. However, a minority demonstrates ambiguous features and continues to be a diagnostic challenge. Genetic expression profiling (GEP) assays have been developed in an effort to resolve this dilemma. These assays measure mRNA levels of specified genes using reverse transcription quantitative polymerase chain reaction technology. The development of GEP assays, methodology, challenges associated with GEP validation and testing, and the suitability of a currently available GEP test for clinical use are reviewed.",
keywords = "Diagnostic test, Genetic expression profiling, GEP, Melanoma, RT-PCR",
author = "Sancy Leachman and {Mengden Koon}, Stephanie and Veselina Korcheva and Kevin White",
year = "2017",
doi = "10.1016/j.det.2017.06.016",
language = "English (US)",
journal = "Dermatologic Clinics",
issn = "0733-8635",
publisher = "W.B. Saunders Ltd",

}

TY - JOUR

T1 - Assessing Genetic Expression Profiles in Melanoma Diagnosis

AU - Leachman, Sancy

AU - Mengden Koon, Stephanie

AU - Korcheva, Veselina

AU - White, Kevin

PY - 2017

Y1 - 2017

N2 - Most melanocytic tumors can be characterized as a benign nevus or a melanoma by a trained pathologist using traditional histopathological methods. However, a minority demonstrates ambiguous features and continues to be a diagnostic challenge. Genetic expression profiling (GEP) assays have been developed in an effort to resolve this dilemma. These assays measure mRNA levels of specified genes using reverse transcription quantitative polymerase chain reaction technology. The development of GEP assays, methodology, challenges associated with GEP validation and testing, and the suitability of a currently available GEP test for clinical use are reviewed.

AB - Most melanocytic tumors can be characterized as a benign nevus or a melanoma by a trained pathologist using traditional histopathological methods. However, a minority demonstrates ambiguous features and continues to be a diagnostic challenge. Genetic expression profiling (GEP) assays have been developed in an effort to resolve this dilemma. These assays measure mRNA levels of specified genes using reverse transcription quantitative polymerase chain reaction technology. The development of GEP assays, methodology, challenges associated with GEP validation and testing, and the suitability of a currently available GEP test for clinical use are reviewed.

KW - Diagnostic test

KW - Genetic expression profiling

KW - GEP

KW - Melanoma

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=85026878285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026878285&partnerID=8YFLogxK

U2 - 10.1016/j.det.2017.06.016

DO - 10.1016/j.det.2017.06.016

M3 - Article

JO - Dermatologic Clinics

JF - Dermatologic Clinics

SN - 0733-8635

ER -