Assembly and composition of intracellular particles formed by Moloney murine leukemia virus

Mark Hansen, Laura Jelinek, Russell S. Jones, Jenny Stegeman-Olsen, Eric Barklis

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Assembly of type C retroviruses such as Moloney murine leukemia virus (M-MuLV) ordinarily occurs at the plasma membranes of infected cells and absolutely requires the particle core precursor protein, Pr65gag. Previously we have shown that Pr65gag is membrane associated and that at least a portion of intracellular Pr65gag protein appears to be routed to the plasma membrane by a vesicular transport pathway. Here we show that intracellular particle formation can occur in M-MuLV-infected cells. M-MuLV immature particles were observed by electron microscopy budding into and within rough endoplasmic reticulum, Golgi, and vacuolar compartments. Biochemical fractionation studies indicated that intracellular Pr65gag was present in nonionic detergent-resistant complexes of greater than 150S. Additionally, viral RNA and polymerase functions appeared to be associated with intracellular particles, as were Gag-β-galactosidase fusion proteins which have the capacity to be incorporated into virions. Immature intracellular particles in postnuclear lysates could be proteolytically processed in vitro to mature forms, while extracellular immature M-MuLV particles remained immature as long as 10 h during incubations. The occurrence of M-MuLV-derived intracellular particles demonstrates that Pr65gag can associate with intracellular membranes and indicates that if a plasma membrane Pr65gag receptor exists, it also can be found in other membrane compartments. These results support the hypothesis that intracellular particles may serve as a virus reservior during in vivo infections.

Original languageEnglish (US)
Pages (from-to)5163-5174
Number of pages12
JournalJournal of Virology
Volume67
Issue number9
StatePublished - Sep 1993

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Murine leukemia virus
Moloney murine leukemia virus
Virion
Cell Membrane
immatures
plasma membrane
Galactosidases
virion
Intracellular Membranes
Protein Precursors
Membranes
Rough Endoplasmic Reticulum
Retroviridae
Viral RNA
DNA-Directed RNA Polymerases
Detergents
galactosidases
proteins
Electron Microscopy
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Hansen, M., Jelinek, L., Jones, R. S., Stegeman-Olsen, J., & Barklis, E. (1993). Assembly and composition of intracellular particles formed by Moloney murine leukemia virus. Journal of Virology, 67(9), 5163-5174.

Assembly and composition of intracellular particles formed by Moloney murine leukemia virus. / Hansen, Mark; Jelinek, Laura; Jones, Russell S.; Stegeman-Olsen, Jenny; Barklis, Eric.

In: Journal of Virology, Vol. 67, No. 9, 09.1993, p. 5163-5174.

Research output: Contribution to journalArticle

Hansen, M, Jelinek, L, Jones, RS, Stegeman-Olsen, J & Barklis, E 1993, 'Assembly and composition of intracellular particles formed by Moloney murine leukemia virus', Journal of Virology, vol. 67, no. 9, pp. 5163-5174.
Hansen, Mark ; Jelinek, Laura ; Jones, Russell S. ; Stegeman-Olsen, Jenny ; Barklis, Eric. / Assembly and composition of intracellular particles formed by Moloney murine leukemia virus. In: Journal of Virology. 1993 ; Vol. 67, No. 9. pp. 5163-5174.
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