Assay of Rab25 function in ovarian and breast cancers

Kwai Wa Cheng, Yiling Lu, Gordon Mills

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

There is a multitude of critical steps during the pathogenesis of cancer that allow cells to acquire the ability to escape from normal controls on cell growth, to avoid programmed cell death, and to become malignant. Here, we describe a molecular approach that can be broadly applied to identify drivers of genomic aberrations in cancer development. In the process, areas of genomic aberrations and genes that are dysregulated by genomic amplification are identified by array comparative genomic hybridization (CGH) and transcription profiling, respectively, with major emphasis on coordinating amplification at the CGH and RNA level and on correlation with patient's outcomes. Once candidate genes are identified, we perform functional genomics by manipulating levels in normal and tumor cells using RNAi or transfection, and assessing a battery of cellular functions including proliferation, anti-apoptosis, loss of contact inhibition, changes in cell signaling or transcriptional profiles, anchorage-independent growth, and in vivo tumor growth. We have successfully used this approach to identify the RAB25 gene that has been implicated in the progression and aggressiveness of ovarian and breast cancers.

Original languageEnglish (US)
Article number17
Pages (from-to)202-215
Number of pages14
JournalMethods in Enzymology
Volume403
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

Fingerprint

Ovarian Neoplasms
Assays
Genes
Breast Neoplasms
Aberrations
Comparative Genomic Hybridization
Amplification
Tumors
Cell signaling
Neoplasms
Growth
Cell growth
Cell death
Transcription
Contact Inhibition
Genomics
RNA Interference
Cells
RNA
Apoptosis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Assay of Rab25 function in ovarian and breast cancers. / Cheng, Kwai Wa; Lu, Yiling; Mills, Gordon.

In: Methods in Enzymology, Vol. 403, 17, 01.12.2005, p. 202-215.

Research output: Contribution to journalArticle

Cheng, Kwai Wa ; Lu, Yiling ; Mills, Gordon. / Assay of Rab25 function in ovarian and breast cancers. In: Methods in Enzymology. 2005 ; Vol. 403. pp. 202-215.
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