Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs

Anton K. Raap, Marja J M Van Der Burg, Jeroen Knijnenburg, Eric Meershoek, Carla Rosenberg, Joe Gray, Joop Wiegant, J. Graeme Hodgson, Hans J. Tanke

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Fluorescent cis-platinum compounds that react with the N7 atom of guanine are useful for labeling nucleic acids in fluorescence hybridization applications. Here we report that cyanin (CyN) cis-platinum labeling of DNA samples for array comparative genomic hybridizations (arrayCGH) can be achieved reproducibly and reliably. We demonstrate that degrees of labeling of approximately 1% of all nucleotides in test and reference DNA samples with CyN3- and CyN5-cis-platinum produces arrayCGH signal-to-background ratios ranging from 30 to 40. The arrayCGH results achieved during analyses of mouse and human tumor samples were comparable to those achieved using enzymatic labeling. Thus, we conclude that Cy-cis-platinum labeling is an alternative to enzymatic labeling for arrayCGH.

Original languageEnglish (US)
Pages (from-to)130-134
Number of pages5
JournalBioTechniques
Volume37
Issue number1
Publication statusPublished - Jul 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Raap, A. K., Van Der Burg, M. J. M., Knijnenburg, J., Meershoek, E., Rosenberg, C., Gray, J., ... Tanke, H. J. (2004). Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs. BioTechniques, 37(1), 130-134.