Aqueous cell differentiation in anterior uveitis using fourier-domain optical coherence tomography

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16 Citations (Scopus)

Abstract

PURPOSE. The differential diagnosis of a patient presenting with anterior uveitis is broad and can present a diagnostic challenge. In this study, we evaluate the characteristic findings of inflammatory cells on optical coherence tomography (OCT) both in vitro and in vivo. METHODS. Blood from two healthy volunteers was prepared using standardized methods for cell sorting with a flow cytometer (FASCAria). Neutrophils, lymphocytes, monocytes, and red blood cells were placed in suspension and scanned with a 26-kHz Fourier-domain OCT system (RTVue) with 5-lm axial resolution. Custom software algorithms were used to identify cells based on their reflectance distribution. These algorithms were then applied to OCT images obtained from uveitis patients with active anterior chamber inflammation. RESULTS. On OCT images the cells appeared as hyperreflective spots. In vitro, cell reflectance was statistically significantly different between all of the cell types (neutrophils, monocytes, lymphocytes, and red blood cells, P <0.001, Mann-Whitney test). In vivo, the relationship between underlying disease and cell type imaged on OCT was highly statistically significant, with human leukocyte antigen (HLA)-B27–associated uveitis patients having a predominantly polymorphonuclear pattern on OCT and sarcoidosis and inflammatory bowel disease patients having a predominantly mononuclear pattern on OCT (P <0.001, Fisher’s exact test). CONCLUSIONS. These in vitro and in vivo data demonstrate the potential of OCT to evaluate cells in the anterior chamber of patients noninvasively. Optical coherence tomography may be a useful adjunct to guide the diagnosis and treatment of ocular inflammatory conditions.

Original languageEnglish (US)
Pages (from-to)1430-1436
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number3
DOIs
StatePublished - 2015

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Anterior Uveitis
Optical Coherence Tomography
Cell Differentiation
Uveitis
Anterior Chamber
Monocytes
Neutrophils
Erythrocytes
Lymphocytes
Sarcoidosis
HLA Antigens
Inflammatory Bowel Diseases
Suspensions
Healthy Volunteers
Differential Diagnosis
Software
Inflammation

Keywords

  • Anterior uveitis
  • Fourier-domain optical coherence tomography
  • Image analysis
  • Mononuclear leukocytes
  • Polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

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title = "Aqueous cell differentiation in anterior uveitis using fourier-domain optical coherence tomography",
abstract = "PURPOSE. The differential diagnosis of a patient presenting with anterior uveitis is broad and can present a diagnostic challenge. In this study, we evaluate the characteristic findings of inflammatory cells on optical coherence tomography (OCT) both in vitro and in vivo. METHODS. Blood from two healthy volunteers was prepared using standardized methods for cell sorting with a flow cytometer (FASCAria). Neutrophils, lymphocytes, monocytes, and red blood cells were placed in suspension and scanned with a 26-kHz Fourier-domain OCT system (RTVue) with 5-lm axial resolution. Custom software algorithms were used to identify cells based on their reflectance distribution. These algorithms were then applied to OCT images obtained from uveitis patients with active anterior chamber inflammation. RESULTS. On OCT images the cells appeared as hyperreflective spots. In vitro, cell reflectance was statistically significantly different between all of the cell types (neutrophils, monocytes, lymphocytes, and red blood cells, P <0.001, Mann-Whitney test). In vivo, the relationship between underlying disease and cell type imaged on OCT was highly statistically significant, with human leukocyte antigen (HLA)-B27–associated uveitis patients having a predominantly polymorphonuclear pattern on OCT and sarcoidosis and inflammatory bowel disease patients having a predominantly mononuclear pattern on OCT (P <0.001, Fisher’s exact test). CONCLUSIONS. These in vitro and in vivo data demonstrate the potential of OCT to evaluate cells in the anterior chamber of patients noninvasively. Optical coherence tomography may be a useful adjunct to guide the diagnosis and treatment of ocular inflammatory conditions.",
keywords = "Anterior uveitis, Fourier-domain optical coherence tomography, Image analysis, Mononuclear leukocytes, Polymorphonuclear leukocytes",
author = "Jennifer Rose-Nussbaumer and Yan Li and Phoebe Lin and Eric Suhler and Mark Asquith and Rosenbaum, {James (Jim)} and David Huang",
year = "2015",
doi = "10.1167/iovs.14-15118",
language = "English (US)",
volume = "56",
pages = "1430--1436",
journal = "Investigative Ophthalmology and Visual Science",
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publisher = "Association for Research in Vision and Ophthalmology Inc.",
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T1 - Aqueous cell differentiation in anterior uveitis using fourier-domain optical coherence tomography

AU - Rose-Nussbaumer, Jennifer

AU - Li, Yan

AU - Lin, Phoebe

AU - Suhler, Eric

AU - Asquith, Mark

AU - Rosenbaum, James (Jim)

AU - Huang, David

PY - 2015

Y1 - 2015

N2 - PURPOSE. The differential diagnosis of a patient presenting with anterior uveitis is broad and can present a diagnostic challenge. In this study, we evaluate the characteristic findings of inflammatory cells on optical coherence tomography (OCT) both in vitro and in vivo. METHODS. Blood from two healthy volunteers was prepared using standardized methods for cell sorting with a flow cytometer (FASCAria). Neutrophils, lymphocytes, monocytes, and red blood cells were placed in suspension and scanned with a 26-kHz Fourier-domain OCT system (RTVue) with 5-lm axial resolution. Custom software algorithms were used to identify cells based on their reflectance distribution. These algorithms were then applied to OCT images obtained from uveitis patients with active anterior chamber inflammation. RESULTS. On OCT images the cells appeared as hyperreflective spots. In vitro, cell reflectance was statistically significantly different between all of the cell types (neutrophils, monocytes, lymphocytes, and red blood cells, P <0.001, Mann-Whitney test). In vivo, the relationship between underlying disease and cell type imaged on OCT was highly statistically significant, with human leukocyte antigen (HLA)-B27–associated uveitis patients having a predominantly polymorphonuclear pattern on OCT and sarcoidosis and inflammatory bowel disease patients having a predominantly mononuclear pattern on OCT (P <0.001, Fisher’s exact test). CONCLUSIONS. These in vitro and in vivo data demonstrate the potential of OCT to evaluate cells in the anterior chamber of patients noninvasively. Optical coherence tomography may be a useful adjunct to guide the diagnosis and treatment of ocular inflammatory conditions.

AB - PURPOSE. The differential diagnosis of a patient presenting with anterior uveitis is broad and can present a diagnostic challenge. In this study, we evaluate the characteristic findings of inflammatory cells on optical coherence tomography (OCT) both in vitro and in vivo. METHODS. Blood from two healthy volunteers was prepared using standardized methods for cell sorting with a flow cytometer (FASCAria). Neutrophils, lymphocytes, monocytes, and red blood cells were placed in suspension and scanned with a 26-kHz Fourier-domain OCT system (RTVue) with 5-lm axial resolution. Custom software algorithms were used to identify cells based on their reflectance distribution. These algorithms were then applied to OCT images obtained from uveitis patients with active anterior chamber inflammation. RESULTS. On OCT images the cells appeared as hyperreflective spots. In vitro, cell reflectance was statistically significantly different between all of the cell types (neutrophils, monocytes, lymphocytes, and red blood cells, P <0.001, Mann-Whitney test). In vivo, the relationship between underlying disease and cell type imaged on OCT was highly statistically significant, with human leukocyte antigen (HLA)-B27–associated uveitis patients having a predominantly polymorphonuclear pattern on OCT and sarcoidosis and inflammatory bowel disease patients having a predominantly mononuclear pattern on OCT (P <0.001, Fisher’s exact test). CONCLUSIONS. These in vitro and in vivo data demonstrate the potential of OCT to evaluate cells in the anterior chamber of patients noninvasively. Optical coherence tomography may be a useful adjunct to guide the diagnosis and treatment of ocular inflammatory conditions.

KW - Anterior uveitis

KW - Fourier-domain optical coherence tomography

KW - Image analysis

KW - Mononuclear leukocytes

KW - Polymorphonuclear leukocytes

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