Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR

T. A. Gonwa, Louis Picker, H. V. Raff, S. M. Goyert, J. Silver, J. D. Stobo

Research output: Contribution to journalArticle

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Abstract

Utilizing a monoclonal antibody (Mac-120) specific for 40 to 60% of peripheral blood adherent mononuclear cells (M∅), we were able to separate M∅ into two populations based on their reactivity with the antibody. Both populations, Mac-120 + and Mac-120 - cells, were then compared for a) their ability to present antigen to T cells, b) their display of HLA-DR determinants, c) their ability to stimulate in an autologous and allogeneic mixed lymphocyte, and d) their display of an Ia molecule, HLA-DS, which is distinct from HLA-DR and which is homologous with murine I-A. Our findings indicate that a) only Mac-120 + cells can present antigen, b) Mac-120 + and Mac-120 - cell populations are equivalent in terms of the number of HLA-DR + cells and in the mean density of HLA-DR determinants per cell, c) although Mac-120 + and Mac-120 - cells are equivalent in their ability to serve as stimulators in an allogeneic mixed lymphocyte reaction, Mac-120 + cells are better stimulators in an autologous mixed lymphocyte reaction, and d) only Mac-120 + cells display HLA-DS. These studies demonstrate that peripheral adherent mononuclear cells exhibit heterogeneity with regard to their display of Ia antigens. Furthermore, they provide functional data to support the existence of a human Ia determinant, HLA-DS, which is distinct from HLA-DR and which is important in antigen presentation and stimulation in the autologous mixed lymphocyte reaction.

Original languageEnglish (US)
Pages (from-to)706-711
Number of pages6
JournalJournal of Immunology
Volume130
Issue number2
StatePublished - 1983
Externally publishedYes

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HLA-DQ Antigens
HLA-DR Antigens
Monocytes
Antigens
Mixed Lymphocyte Culture Test
Population
Histocompatibility Antigens Class II
Antigen Presentation
Monoclonal Antibodies
Lymphocytes

ASJC Scopus subject areas

  • Immunology

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Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. / Gonwa, T. A.; Picker, Louis; Raff, H. V.; Goyert, S. M.; Silver, J.; Stobo, J. D.

In: Journal of Immunology, Vol. 130, No. 2, 1983, p. 706-711.

Research output: Contribution to journalArticle

Gonwa, T. A. ; Picker, Louis ; Raff, H. V. ; Goyert, S. M. ; Silver, J. ; Stobo, J. D. / Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. In: Journal of Immunology. 1983 ; Vol. 130, No. 2. pp. 706-711.
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T1 - Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR

AU - Gonwa, T. A.

AU - Picker, Louis

AU - Raff, H. V.

AU - Goyert, S. M.

AU - Silver, J.

AU - Stobo, J. D.

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N2 - Utilizing a monoclonal antibody (Mac-120) specific for 40 to 60% of peripheral blood adherent mononuclear cells (M∅), we were able to separate M∅ into two populations based on their reactivity with the antibody. Both populations, Mac-120 + and Mac-120 - cells, were then compared for a) their ability to present antigen to T cells, b) their display of HLA-DR determinants, c) their ability to stimulate in an autologous and allogeneic mixed lymphocyte, and d) their display of an Ia molecule, HLA-DS, which is distinct from HLA-DR and which is homologous with murine I-A. Our findings indicate that a) only Mac-120 + cells can present antigen, b) Mac-120 + and Mac-120 - cell populations are equivalent in terms of the number of HLA-DR + cells and in the mean density of HLA-DR determinants per cell, c) although Mac-120 + and Mac-120 - cells are equivalent in their ability to serve as stimulators in an allogeneic mixed lymphocyte reaction, Mac-120 + cells are better stimulators in an autologous mixed lymphocyte reaction, and d) only Mac-120 + cells display HLA-DS. These studies demonstrate that peripheral adherent mononuclear cells exhibit heterogeneity with regard to their display of Ia antigens. Furthermore, they provide functional data to support the existence of a human Ia determinant, HLA-DS, which is distinct from HLA-DR and which is important in antigen presentation and stimulation in the autologous mixed lymphocyte reaction.

AB - Utilizing a monoclonal antibody (Mac-120) specific for 40 to 60% of peripheral blood adherent mononuclear cells (M∅), we were able to separate M∅ into two populations based on their reactivity with the antibody. Both populations, Mac-120 + and Mac-120 - cells, were then compared for a) their ability to present antigen to T cells, b) their display of HLA-DR determinants, c) their ability to stimulate in an autologous and allogeneic mixed lymphocyte, and d) their display of an Ia molecule, HLA-DS, which is distinct from HLA-DR and which is homologous with murine I-A. Our findings indicate that a) only Mac-120 + cells can present antigen, b) Mac-120 + and Mac-120 - cell populations are equivalent in terms of the number of HLA-DR + cells and in the mean density of HLA-DR determinants per cell, c) although Mac-120 + and Mac-120 - cells are equivalent in their ability to serve as stimulators in an allogeneic mixed lymphocyte reaction, Mac-120 + cells are better stimulators in an autologous mixed lymphocyte reaction, and d) only Mac-120 + cells display HLA-DS. These studies demonstrate that peripheral adherent mononuclear cells exhibit heterogeneity with regard to their display of Ia antigens. Furthermore, they provide functional data to support the existence of a human Ia determinant, HLA-DS, which is distinct from HLA-DR and which is important in antigen presentation and stimulation in the autologous mixed lymphocyte reaction.

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