Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon

Willy Ramos, Jesús Díaz, Ericson L. Gutierrez, Jose S. Lazarte, Mary C. Bohnett, Gerardo Ronceros, Alex Ortega Loayza

Research output: Contribution to journalArticle

Abstract

Background: The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. Materials and methods: Cross-sectional study. The study population included persons of any age or gender, clinically healthy, who were evaluated by a dermatologist to confirm the absence of blistering diseases. Blood samples were analyzed by indirect immunofluorescence (IIF), immunoprecipitation (IP), anti-Dsg1 IgM antibody (Ab) enzyme-linked immunosorbent assay (ELISA), as well as anti-Dsg1 and anti-Dsg3 IgG Ab ELISA. Results: Participants included 21 healthy subjects comprised of 61.9% males and 38.1% females; 47.6% had a positive anti-Dsg1 Ab ELISA for total IgG (or any subclasses). IIF detected antibodies against intercellular spaces in one subject. Anti-Dsg1 Ab IP was mildly positive in 33.3% of the subjects. Anti-Dsg1 IgG subclasses found positive were: IgG1 (19.0%), IgG2 (33.3%), and IgG3 (28.6%); none of the samples were positive for anti-Dsg1 Ab IgM ELISA, and 23.8% of the subjects were positive for anti-Dsg3 Ab ELISA. The age distribution was similar for subjects positive for anti-Dsg1 and anti-Dsg3 Ab ELISA, with higher frequencies found among the 20–29 and 40–49 year-old age groups. Conclusion: A fraction of healthy subjects of the high Peruvian Amazon developed anti-Dsg1 and anti-Dsg3 antibodies, demonstrating the possible presence of environmental factors for endemic pemphigus (EP) at a higher altitude than previously described.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalInternational Journal of Dermatology
Volume57
Issue number3
DOIs
StatePublished - Mar 1 2018

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Anti-Idiotypic Antibodies
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Antibodies
Immunoglobulin G
Population
Indirect Fluorescent Antibody Technique
Immunoprecipitation
Amazona
Trigger Points
Pemphigus
Age Distribution
Extracellular Space
Immunoglobulin M
Age Groups
Cross-Sectional Studies
anti-IgG

ASJC Scopus subject areas

  • Dermatology

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Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon. / Ramos, Willy; Díaz, Jesús; Gutierrez, Ericson L.; Lazarte, Jose S.; Bohnett, Mary C.; Ronceros, Gerardo; Ortega Loayza, Alex.

In: International Journal of Dermatology, Vol. 57, No. 3, 01.03.2018, p. 344-348.

Research output: Contribution to journalArticle

Ramos, Willy ; Díaz, Jesús ; Gutierrez, Ericson L. ; Lazarte, Jose S. ; Bohnett, Mary C. ; Ronceros, Gerardo ; Ortega Loayza, Alex. / Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon. In: International Journal of Dermatology. 2018 ; Vol. 57, No. 3. pp. 344-348.
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abstract = "Background: The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. Materials and methods: Cross-sectional study. The study population included persons of any age or gender, clinically healthy, who were evaluated by a dermatologist to confirm the absence of blistering diseases. Blood samples were analyzed by indirect immunofluorescence (IIF), immunoprecipitation (IP), anti-Dsg1 IgM antibody (Ab) enzyme-linked immunosorbent assay (ELISA), as well as anti-Dsg1 and anti-Dsg3 IgG Ab ELISA. Results: Participants included 21 healthy subjects comprised of 61.9{\%} males and 38.1{\%} females; 47.6{\%} had a positive anti-Dsg1 Ab ELISA for total IgG (or any subclasses). IIF detected antibodies against intercellular spaces in one subject. Anti-Dsg1 Ab IP was mildly positive in 33.3{\%} of the subjects. Anti-Dsg1 IgG subclasses found positive were: IgG1 (19.0{\%}), IgG2 (33.3{\%}), and IgG3 (28.6{\%}); none of the samples were positive for anti-Dsg1 Ab IgM ELISA, and 23.8{\%} of the subjects were positive for anti-Dsg3 Ab ELISA. The age distribution was similar for subjects positive for anti-Dsg1 and anti-Dsg3 Ab ELISA, with higher frequencies found among the 20–29 and 40–49 year-old age groups. Conclusion: A fraction of healthy subjects of the high Peruvian Amazon developed anti-Dsg1 and anti-Dsg3 antibodies, demonstrating the possible presence of environmental factors for endemic pemphigus (EP) at a higher altitude than previously described.",
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T1 - Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon

AU - Ramos, Willy

AU - Díaz, Jesús

AU - Gutierrez, Ericson L.

AU - Lazarte, Jose S.

AU - Bohnett, Mary C.

AU - Ronceros, Gerardo

AU - Ortega Loayza, Alex

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N2 - Background: The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. Materials and methods: Cross-sectional study. The study population included persons of any age or gender, clinically healthy, who were evaluated by a dermatologist to confirm the absence of blistering diseases. Blood samples were analyzed by indirect immunofluorescence (IIF), immunoprecipitation (IP), anti-Dsg1 IgM antibody (Ab) enzyme-linked immunosorbent assay (ELISA), as well as anti-Dsg1 and anti-Dsg3 IgG Ab ELISA. Results: Participants included 21 healthy subjects comprised of 61.9% males and 38.1% females; 47.6% had a positive anti-Dsg1 Ab ELISA for total IgG (or any subclasses). IIF detected antibodies against intercellular spaces in one subject. Anti-Dsg1 Ab IP was mildly positive in 33.3% of the subjects. Anti-Dsg1 IgG subclasses found positive were: IgG1 (19.0%), IgG2 (33.3%), and IgG3 (28.6%); none of the samples were positive for anti-Dsg1 Ab IgM ELISA, and 23.8% of the subjects were positive for anti-Dsg3 Ab ELISA. The age distribution was similar for subjects positive for anti-Dsg1 and anti-Dsg3 Ab ELISA, with higher frequencies found among the 20–29 and 40–49 year-old age groups. Conclusion: A fraction of healthy subjects of the high Peruvian Amazon developed anti-Dsg1 and anti-Dsg3 antibodies, demonstrating the possible presence of environmental factors for endemic pemphigus (EP) at a higher altitude than previously described.

AB - Background: The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. Materials and methods: Cross-sectional study. The study population included persons of any age or gender, clinically healthy, who were evaluated by a dermatologist to confirm the absence of blistering diseases. Blood samples were analyzed by indirect immunofluorescence (IIF), immunoprecipitation (IP), anti-Dsg1 IgM antibody (Ab) enzyme-linked immunosorbent assay (ELISA), as well as anti-Dsg1 and anti-Dsg3 IgG Ab ELISA. Results: Participants included 21 healthy subjects comprised of 61.9% males and 38.1% females; 47.6% had a positive anti-Dsg1 Ab ELISA for total IgG (or any subclasses). IIF detected antibodies against intercellular spaces in one subject. Anti-Dsg1 Ab IP was mildly positive in 33.3% of the subjects. Anti-Dsg1 IgG subclasses found positive were: IgG1 (19.0%), IgG2 (33.3%), and IgG3 (28.6%); none of the samples were positive for anti-Dsg1 Ab IgM ELISA, and 23.8% of the subjects were positive for anti-Dsg3 Ab ELISA. The age distribution was similar for subjects positive for anti-Dsg1 and anti-Dsg3 Ab ELISA, with higher frequencies found among the 20–29 and 40–49 year-old age groups. Conclusion: A fraction of healthy subjects of the high Peruvian Amazon developed anti-Dsg1 and anti-Dsg3 antibodies, demonstrating the possible presence of environmental factors for endemic pemphigus (EP) at a higher altitude than previously described.

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