Anti-idiotypic antisera raised against monoclonal antibody specific for a p24 gag region epitope detects a common interspecies idiotype associated with anti-HIV responses

W. J W Morrow, I. Gaston, T. Anderson, Nancy Haigwood, M. S. McGrath, J. Rosen, K. S. Steimer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

One potential strategy for the control of human immunodeficiency virus (HIV) infection is immune network manipulation using anti-idiotypic antibodies: this study was undertaken to demonstrate experimentally the potential of such an approach which, in a more highly evolved form, could be used for the treatment of the acquired immune deficiency virus (AIDS) and related disorders. Anti-idiotypic antibodies were generated in rabbits against a murine monoclonal antibody identifying an epitope on the p24 gag core protein of HIV. After extensive absorption on affinity columns to remove isotype- and allotype-specific antibodies, the purified anti-idiotypic antibody preparation was shown to have specific complementarity with the immunizing mouse monoclonal antibody. This anti-idiotypic antibody was also shown to recognize a common idiotype associated with HIV-specific antibodies from both humans and chimpanzees infected with the AIDS virus. In addition a group of rats immunized with the anti-Id responded with significant antibody titers to recombinant derived p24 gag. These data indicate that at least a subpopulation of these polyclonal anti-Id antibodies structurally mimics an HIV gag region epitope and suggest that immunoregulation by anti-idiotypic antibodies may have therapeutic utility for the AIDS epidemic.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalViral Immunology
Volume3
Issue number2
StatePublished - 1990
Externally publishedYes

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Immune Sera
Epitopes
Anti-Idiotypic Antibodies
Monoclonal Antibodies
HIV
Viruses
Human Immunodeficiency Virus gag Gene Products
Antibodies
Pan troglodytes
Virus Diseases
Rabbits
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Anti-idiotypic antisera raised against monoclonal antibody specific for a p24 gag region epitope detects a common interspecies idiotype associated with anti-HIV responses. / Morrow, W. J W; Gaston, I.; Anderson, T.; Haigwood, Nancy; McGrath, M. S.; Rosen, J.; Steimer, K. S.

In: Viral Immunology, Vol. 3, No. 2, 1990, p. 99-109.

Research output: Contribution to journalArticle

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