Annexin 2 has an essential role in actin-based macropinocytic rocketing

Christien J. Merrifield, Ursula Rescher, Wolfhard Almers, Jezabel Proust, Volker Gerke, Antonio S. Sechi, Stephen E. Moss

Research output: Contribution to journalArticle

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Abstract

Annexin 2 is a Ca2+ binding protein that binds to and aggregates secretory vesicles at physiological Ca2+ levels [1] and that also associates Ca2+ independently with early endosomes [2, 3]. These properties suggest roles in both exocytosis and endocytosis, but little is known of the dynamics of Annexin 2 distribution in live cells during these processes. We have used evanescent field microscopy to image Annexin 2-GFP in live, secreting rat basophilic leukemia cells and in cells performing pinocytosis. Although we found no evidence of Annexin 2 involvement in exocytosis, we observed an enrichment of Annexin 2-GFP in actin tails propeling macropinosomes. The association of Annexin 2-GFP with rocketing macropinosomes was specific because Annexin 2-GFP was absent from the actin tails of rocketing Listeria. This finding suggests that the association of Annexin 2 with macropinocytic rockets requires native pinosomal membrane. Annexin 2 is necessary for the formation of macropinocytic rockets since overexpression of a dominant-negative Annexin 2 construct abolished the formation of these structures. The same construct did not prevent the movement of Listeria in infected cells. These results show that recruitment of Annexin 2 to nascent macropinosome membranes is an essential prerequisite for actin polymerization-dependent vesicle locomotion.

Original languageEnglish (US)
Pages (from-to)1136-1141
Number of pages6
JournalCurrent Biology
Volume11
Issue number14
DOIs
StatePublished - Jul 24 2001

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Annexins
actin
Actins
exocytosis
endocytosis
Listeria
calcium
tail
cells
endosomes
secretory granules
protein aggregates
leukemia
polymerization
locomotion
binding proteins
microscopy
Exocytosis
Rockets
Tail

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Merrifield, C. J., Rescher, U., Almers, W., Proust, J., Gerke, V., Sechi, A. S., & Moss, S. E. (2001). Annexin 2 has an essential role in actin-based macropinocytic rocketing. Current Biology, 11(14), 1136-1141. https://doi.org/10.1016/S0960-9822(01)00321-9

Annexin 2 has an essential role in actin-based macropinocytic rocketing. / Merrifield, Christien J.; Rescher, Ursula; Almers, Wolfhard; Proust, Jezabel; Gerke, Volker; Sechi, Antonio S.; Moss, Stephen E.

In: Current Biology, Vol. 11, No. 14, 24.07.2001, p. 1136-1141.

Research output: Contribution to journalArticle

Merrifield, CJ, Rescher, U, Almers, W, Proust, J, Gerke, V, Sechi, AS & Moss, SE 2001, 'Annexin 2 has an essential role in actin-based macropinocytic rocketing', Current Biology, vol. 11, no. 14, pp. 1136-1141. https://doi.org/10.1016/S0960-9822(01)00321-9
Merrifield CJ, Rescher U, Almers W, Proust J, Gerke V, Sechi AS et al. Annexin 2 has an essential role in actin-based macropinocytic rocketing. Current Biology. 2001 Jul 24;11(14):1136-1141. https://doi.org/10.1016/S0960-9822(01)00321-9
Merrifield, Christien J. ; Rescher, Ursula ; Almers, Wolfhard ; Proust, Jezabel ; Gerke, Volker ; Sechi, Antonio S. ; Moss, Stephen E. / Annexin 2 has an essential role in actin-based macropinocytic rocketing. In: Current Biology. 2001 ; Vol. 11, No. 14. pp. 1136-1141.
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