Annexin 2 has an essential role in actin-based macropinocytic rocketing

Christien J. Merrifield, Ursula Rescher, Wolfhard Almers, Jezabel Proust, Volker Gerke, Antonio S. Sechi, Stephen E. Moss

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Annexin 2 is a Ca2+ binding protein that binds to and aggregates secretory vesicles at physiological Ca2+ levels [1] and that also associates Ca2+ independently with early endosomes [2, 3]. These properties suggest roles in both exocytosis and endocytosis, but little is known of the dynamics of Annexin 2 distribution in live cells during these processes. We have used evanescent field microscopy to image Annexin 2-GFP in live, secreting rat basophilic leukemia cells and in cells performing pinocytosis. Although we found no evidence of Annexin 2 involvement in exocytosis, we observed an enrichment of Annexin 2-GFP in actin tails propeling macropinosomes. The association of Annexin 2-GFP with rocketing macropinosomes was specific because Annexin 2-GFP was absent from the actin tails of rocketing Listeria. This finding suggests that the association of Annexin 2 with macropinocytic rockets requires native pinosomal membrane. Annexin 2 is necessary for the formation of macropinocytic rockets since overexpression of a dominant-negative Annexin 2 construct abolished the formation of these structures. The same construct did not prevent the movement of Listeria in infected cells. These results show that recruitment of Annexin 2 to nascent macropinosome membranes is an essential prerequisite for actin polymerization-dependent vesicle locomotion.

Original languageEnglish (US)
Pages (from-to)1136-1141
Number of pages6
JournalCurrent Biology
Volume11
Issue number14
DOIs
StatePublished - Jul 24 2001

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Merrifield, C. J., Rescher, U., Almers, W., Proust, J., Gerke, V., Sechi, A. S., & Moss, S. E. (2001). Annexin 2 has an essential role in actin-based macropinocytic rocketing. Current Biology, 11(14), 1136-1141. https://doi.org/10.1016/S0960-9822(01)00321-9