Anglerfish islet pre-proglucagon II: Nucleotide and corresponding amino acid sequence of the cDNA

P. K. Lund, Richard Goodman, M. R. Montminy, P. C. Dee, J. F. Habener

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Glucagon is a 29-amino acid peptide hormone that regulates blood glucose concentrations. It is a member of a family of structurally related hormones that includes in addition to glucagon, vasoactive intestinal peptide, gastric inhibitory peptide, and secretin. Like other peptide hormones, glucagon is synthesized as a larger precursor. Previously, we reported the amino acid sequence of an anglerfish pre-proglucagon of apparent M(r) = 14,500, derived from the sequence of cloned cDNAs. Now we have determined the nucleotide sequence of cDNAs encoding a separate anglerfish preproglucagon of apparent M(r) = 12,500 and have derived the complete amino acid sequence of this second precursor. The configurations of the two pre-proglucagons are similar. Each pre-proglucagon is a polyprotein that contains two glucagon-related peptides arranged in tandem, a 29-amino acid glucagon sequence and a 34-amino acid sequence which shows homology to glucagon and the other members of the glucagon family. These two peptides are linked in the precursors by lysine-arginine and intervening penta- or tetrapeptides. The glucagon sequences of 29 amino acids in the two precursors are closely homologous. Similarly, the 34-amino acid peptide sequences in the two precursors are highly homologous. Analyses of the genomic DNA prepared from the spleen of a single anglerfish show that these two pre-proglucagons are encoded by at least two separate genes. Analyses of the mRNAs in the anglerfish islets indicate that a single mRNA species encodes a M(r) = 14,500 pre-proglucagon but suggest that two separate mRNAs contain coding sequences for the M(r) = 12,500 pre-proglucagon. These studies indicate that in the anglerfish, glucagon is synthesized by way of the expression of at least two genes.

Original languageEnglish (US)
Pages (from-to)3280-3284
Number of pages5
JournalJournal of Biological Chemistry
Volume258
Issue number5
StatePublished - 1983
Externally publishedYes

Fingerprint

Proglucagon
Glucagon
Amino Acid Sequence
Nucleotides
Complementary DNA
Amino Acids
Peptides
Peptide Hormones
Messenger RNA
Genes
Polyproteins
Rubiaceae
Secretin
Vasoactive Intestinal Peptide
Lysine
Arginine
Blood Glucose
Stomach
Spleen
Hormones

ASJC Scopus subject areas

  • Biochemistry

Cite this

Anglerfish islet pre-proglucagon II : Nucleotide and corresponding amino acid sequence of the cDNA. / Lund, P. K.; Goodman, Richard; Montminy, M. R.; Dee, P. C.; Habener, J. F.

In: Journal of Biological Chemistry, Vol. 258, No. 5, 1983, p. 3280-3284.

Research output: Contribution to journalArticle

Lund, P. K. ; Goodman, Richard ; Montminy, M. R. ; Dee, P. C. ; Habener, J. F. / Anglerfish islet pre-proglucagon II : Nucleotide and corresponding amino acid sequence of the cDNA. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 5. pp. 3280-3284.
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