Abstract
Objective - We evaluated the role of angiotensin II (AII) in a marrow-derived macrophage-driven model of atherosclerosis. Methods and Results - Eight-week-old C57BL/6 wild-type mice were reconstituted with bone marrow harvested from apolipoprotein E-deficient (apoE-/- → apoE+/+) or wild-type for apoE gene (apoE+/+ → apoE+/+) mice. At 20 weeks, mice were exposed to either AII (1000 ng/kg per minute subcutaneously) or saline for 2 weeks. Animals did not differ in body weight, blood pressure, cholesterol/triglycerides, or peripheral blood monocyte counts. ApoE-/- → apoE+/+ mice exposed to AII had 3-fold greater atherosclerotic area than saline-treated apoE-/- → apoE+/+ mice. By contrast, AII did not affect atherosclerosis in apoE+/+ → apoE+/+ mice. Macrophage-positive areas were increased by AII in mice reconstituted with either apoE-deficient or apoE-competent marrow. AII also significantly increased fragmentation of elastin laminae in both apoE-/- → apoE+/+ and apoE+/+ → apoE+/+ mice. In vitro, AII caused greater increase in monocyte chemoattractant protein-1-stimulated migration of macrophages harvested from AII-infused versus saline-infused mice. Conclusions - The current studies reveal that AII has both initiating and sustaining proatherogenic effects. By promoting macrophage migration into the vascular intima, AII is pivotal in initiating atherosclerosis; by promoting elastin breaks, a novel mechanism implicated in migration and proliferation of smooth muscle cells, AII may be pivotal in subsequent development and expansion of atherosclerotic lesion.
Original language | English (US) |
---|---|
Pages (from-to) | 2143-2148 |
Number of pages | 6 |
Journal | Arteriosclerosis, thrombosis, and vascular biology |
Volume | 24 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2004 |
Externally published | Yes |
Keywords
- Angiotensin II
- Atherosclerosis
- Macrophage
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine