Androgen receptor and 5α-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques

Charles Roselli, N. B. West, R. M. Brenner

Research output: Contribution to journalArticle

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Abstract

We measured androgen receptors (AR) and 5α-reductase activity (5αRA) in the ductuli efferentes and epididymides from adult rhesus macaques. Tissue samples were either assayed biochemically for AR or stained immunocytochemically (ICC) with a monoclonal antibody against AR. To estimate 5αRA, tissue microsomes were incubated with [1α,2α-3H]testosterone, and the [3H]dihydrotestosterone formed was quantified. We found significant regional differences in the levels of both 5αRA and AR in the excurrent ducts. In general, both enzyme activity and AR levels were higher in the caput and corpus epididymis than in ductuli efferentes and cauda epididymis. With ICC, positive nuclear AR staining was detected in all epithelial cell types, whereas variable numbers of stromal cells were positively stained. Our data demonstrate that there are segmental differences in the concentrations of 5αRA and AR in epididymis and suggest that there may be regional differences in the regulation of epididymal functions by androgen.

Original languageEnglish (US)
Pages (from-to)739-745
Number of pages7
JournalBiology of Reproduction
Volume44
Issue number4
StatePublished - 1991

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Epididymis
Androgen Receptors
Macaca mulatta
Oxidoreductases
Dihydrotestosterone
Cytoplasmic and Nuclear Receptors
Stromal Cells
Microsomes
Androgens
Testosterone
Epithelial Cells
Monoclonal Antibodies
Staining and Labeling
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Androgen receptor and 5α-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques. / Roselli, Charles; West, N. B.; Brenner, R. M.

In: Biology of Reproduction, Vol. 44, No. 4, 1991, p. 739-745.

Research output: Contribution to journalArticle

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