Analysis of the promoter region of the cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus

Michiko Nakano, T. Kikuchi, H. Mashiko, H. Ogawara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The appropriate location and orientation of the kanamycin resistance gene (kmr) cloned on multicopy plasmids were determined by subcloning experiments. The transcription start site was identified by high-resolution S1 mapping and the kmr mRNA was shown to have a long leader of about 40 bp. An additional transcript upstream of the kmr gene was also detected, which ran in the opposite direction and overlapped 2 to 3 nucleotides with the kmr transcript. The presumptive promoter region of this physiologically unidentified RNA was similar to the Escherichia coli promoter consensus sequence both in the -10 and -35 regions, whereas the putative promoter region of the kmr gene exhibited sequence similarities in the -10 region to the promoters of the endoglycosidase H (endoH) and the viomycin phosphotransferase (vph) genes from Streptomyces.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalJournal of Antibiotics
Volume42
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

Kanamycin Resistance
Streptomyces
Genetic Promoter Regions
Genes
Glycoside Hydrolases
Transcription Initiation Site
Consensus Sequence
Plasmids
Nucleotides
RNA
Escherichia coli
Messenger RNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Analysis of the promoter region of the cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus. / Nakano, Michiko; Kikuchi, T.; Mashiko, H.; Ogawara, H.

In: Journal of Antibiotics, Vol. 42, No. 3, 1989, p. 413-422.

Research output: Contribution to journalArticle

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