@inbook{ce9b069ec3b44d0c86d9f2311ba6cfab,
title = "Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test",
abstract = "Zika virus (ZIKV) is a newly emerged mosquito-borne flavivirus that has been associated with birth defects of babies born to ZIKV-infected mothers. Neutralization activity of serum derived from ZIKV infected and vaccinated individuals is a critical component for characterizing immune response to infection and vaccine efficacy. This protocol describes a modified plaque reduction neutralization 50 (PRNT50) assay that includes an immunostaining step to improve reproducibility and throughput of the assay.",
keywords = "Antibody, Flavivirus, Focus reduction neutralization, Immunostain, Plaque reduction neutralization, Zika virus",
author = "Smith, {Jessica L.} and Hirsch, {Alec J.}",
note = "Funding Information: The work presented in this manuscript was supported by the Pilot Program support from the Oregon National Primate Research Center (ONPRC) P51-ODO11092, R21-HD091032, and HHSN27201400055C awarded by the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Veterinarian and husbandry staff provide excellent care for the animals used in this study. Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
doi = "10.1007/978-1-0716-0581-3_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "73--80",
booktitle = "Methods in Molecular Biology",
}