Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

Kimberly M. Winges, Donald H. Gilden, Jeffrey L. Bennett, Xiaoli Yu, Alanna M. Ritchie, Gregory P. Owens

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89 ± 2% (mean ± SE) of the CD138+ cell population (P < 0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11 ± 2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.

Original languageEnglish (US)
Pages (from-to)226-234
Number of pages9
JournalJournal of Neuroimmunology
Volume192
Issue number1-2
DOIs
StatePublished - Dec 1 2007

Keywords

  • Multiple sclerosis
  • Plasma blasts
  • Plasma cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts'. Together they form a unique fingerprint.

  • Cite this