Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

Kimberly M. Winges; Donald H. Gilden; Jeffrey L. Bennett; Xiaoli Yu; Alanna M. Ritchie; Gregory P. Owens

doi:10.1016/j.jneuroim.2007.10.009

Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

Kimberly M. Winges, Donald H. Gilden, Jeffrey L. Bennett, Xiaoli Yu, Alanna M. Ritchie, Gregory P. Owens

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89 ± 2% (mean ± SE) of the CD138+ cell population (P < 0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11 ± 2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.

Original language	English (US)
Pages (from-to)	226-234
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	192
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2007.10.009
State	Published - Dec 2007
Externally published	Yes

Keywords

Multiple sclerosis
Plasma blasts
Plasma cells

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2007.10.009

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title = "Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts",

abstract = "Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89 ± 2% (mean ± SE) of the CD138+ cell population (P < 0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11 ± 2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.",

keywords = "Multiple sclerosis, Plasma blasts, Plasma cells",

author = "Winges, {Kimberly M.} and Gilden, {Donald H.} and Bennett, {Jeffrey L.} and Xiaoli Yu and Ritchie, {Alanna M.} and Owens, {Gregory P.}",

note = "Funding Information: This work was supported in part by Public Health Service grant NS 32623 from the National Institutes of Health (D.G.H. and G.P.O.), and research grant 3908 from the National Multiple Sclerosis Society (J.L.B.). Kimberly Winges is the recipient of a Howard Hughes Medical Institute Research Fellowship Award. The authors thank John Corboy, MD, for patient recruitment and CSF collection, Ms. Sydni Edwards for technical help, Ms. Karen Helm for cell sorting, Marina Hoffman for editorial review and Cathy Allen for manuscript preparation. ",

year = "2007",

month = dec,

doi = "10.1016/j.jneuroim.2007.10.009",

language = "English (US)",

volume = "192",

pages = "226--234",

journal = "Journal of Neuroimmunology",

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T1 - Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

AU - Winges, Kimberly M.

AU - Gilden, Donald H.

AU - Bennett, Jeffrey L.

AU - Yu, Xiaoli

AU - Ritchie, Alanna M.

AU - Owens, Gregory P.

N1 - Funding Information: This work was supported in part by Public Health Service grant NS 32623 from the National Institutes of Health (D.G.H. and G.P.O.), and research grant 3908 from the National Multiple Sclerosis Society (J.L.B.). Kimberly Winges is the recipient of a Howard Hughes Medical Institute Research Fellowship Award. The authors thank John Corboy, MD, for patient recruitment and CSF collection, Ms. Sydni Edwards for technical help, Ms. Karen Helm for cell sorting, Marina Hoffman for editorial review and Cathy Allen for manuscript preparation.

PY - 2007/12

Y1 - 2007/12

N2 - Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89 ± 2% (mean ± SE) of the CD138+ cell population (P < 0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11 ± 2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.

AB - Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 showed that CD138+19+ plasma blasts constituted 89 ± 2% (mean ± SE) of the CD138+ cell population (P < 0.00001), with more mature plasma cells (CD138+19-) constituting the remaining 11 ± 2%. Sequence analysis of immunoglobulin variable regions in single CD138+19+ and CD138+19- cells sorted from MS CSF identified many of the same clonal populations in both populations, indicating a continuum of clonally related plasma cell subtypes of which CD138+19+ plasma blasts are most abundant.

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KW - Plasma cells

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Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

Abstract

Keywords

ASJC Scopus subject areas

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