TY - JOUR
T1 - Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes
AU - Chou, Sunwen
AU - Dennison, Karen M.
N1 - Funding Information:
Received 15 November 1990; revised 28 January 1991. Financial support: Department of Veterans Affairs research funds. Reprints orcorrespondence: Dr. Sunwen Chou, VA Medical Center 111F, P.O. Box 1034, Portland, OR972CJ7.
PY - 1991/6
Y1 - 1991/6
N2 - Nucleotide sequences of a part of the envelope glycoprotein B (gB) gene of human cytomegalovirus (CMV), encoding epitopes recognized by virus-neutralizing monoclonal antibodies, were determined for 12 distinct clinical strains of CMV after amplification of suitable templates using the polymerase chain reaction. Sequence analysis of this region (codons 384-717) revealed that the clinical strains and previously sequenced laboratory strains Towne and AD169 belong to one of four variant groups, each with a characteristic nucleotide and peptide sequence. Peptide homology was >99% for strains within a group, and varied from 91% to 98% for strains in different groups. Variation was most frequent between codons 448 and 480. The gB group ofa CMV strain could be determined by restriction analysis of a small target sequence amplified from viral genomic DNA, and an additional 28 clinical strains were grouped in this manner. The existence of a limited number of variants of gB among clinical strains facilitates analysis of biologic function and cross-reactivity of immune responses.
AB - Nucleotide sequences of a part of the envelope glycoprotein B (gB) gene of human cytomegalovirus (CMV), encoding epitopes recognized by virus-neutralizing monoclonal antibodies, were determined for 12 distinct clinical strains of CMV after amplification of suitable templates using the polymerase chain reaction. Sequence analysis of this region (codons 384-717) revealed that the clinical strains and previously sequenced laboratory strains Towne and AD169 belong to one of four variant groups, each with a characteristic nucleotide and peptide sequence. Peptide homology was >99% for strains within a group, and varied from 91% to 98% for strains in different groups. Variation was most frequent between codons 448 and 480. The gB group ofa CMV strain could be determined by restriction analysis of a small target sequence amplified from viral genomic DNA, and an additional 28 clinical strains were grouped in this manner. The existence of a limited number of variants of gB among clinical strains facilitates analysis of biologic function and cross-reactivity of immune responses.
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U2 - 10.1093/infdis/163.6.1229
DO - 10.1093/infdis/163.6.1229
M3 - Article
C2 - 1709960
AN - SCOPUS:0025946399
SN - 0022-1899
VL - 163
SP - 1229
EP - 1234
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -