An inhibitor of the chymotrypsin-like activity of the proteasome (PSI) induces similar morphological changes in various cell lines

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17 Citations (Scopus)

Abstract

Different established cell lines (SK-v human keratinocytes, B16F10 mouse melanoma, Muntjac fibroblasts, 3T3 mouse fibroblasts, CLV human fibroblasts) as well as primary cultures of mouse fibroblasts and giant cells were treated with N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (proteasome inhibitor, PSI), a specific inhibitor of the chymotrypsin-like activity of the proteasome. PSI induced well characterized morphological changes in all cell lines studied, including vacuolization and accumulation of perinuclear aggregates, as detected by amido black staining. Together with previous reports on HeLa cells, the present findings support the hypothesis that ubiquitin- and proteasome-dependent proteolysis does not occurr randomly in the entire cell, but it is concentrated in a well defined perinuclear region, the proteolytic center.

Original languageEnglish (US)
Pages (from-to)211-214
Number of pages4
JournalFolia Histochemica et Cytobiologica
Volume35
Issue number4
StatePublished - 1997
Externally publishedYes

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Proteasome Inhibitors
Chymotrypsin
Proteasome Endopeptidase Complex
Fibroblasts
Cell Line
Amido Black
Muntjacs
Giant Cells
Ubiquitin
Keratinocytes
HeLa Cells
Proteolysis
Melanoma
Staining and Labeling

Keywords

  • Proteasome
  • Proteolysis
  • Proteolytic center
  • Ubiquitin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

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abstract = "Different established cell lines (SK-v human keratinocytes, B16F10 mouse melanoma, Muntjac fibroblasts, 3T3 mouse fibroblasts, CLV human fibroblasts) as well as primary cultures of mouse fibroblasts and giant cells were treated with N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (proteasome inhibitor, PSI), a specific inhibitor of the chymotrypsin-like activity of the proteasome. PSI induced well characterized morphological changes in all cell lines studied, including vacuolization and accumulation of perinuclear aggregates, as detected by amido black staining. Together with previous reports on HeLa cells, the present findings support the hypothesis that ubiquitin- and proteasome-dependent proteolysis does not occurr randomly in the entire cell, but it is concentrated in a well defined perinuclear region, the proteolytic center.",
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AB - Different established cell lines (SK-v human keratinocytes, B16F10 mouse melanoma, Muntjac fibroblasts, 3T3 mouse fibroblasts, CLV human fibroblasts) as well as primary cultures of mouse fibroblasts and giant cells were treated with N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (proteasome inhibitor, PSI), a specific inhibitor of the chymotrypsin-like activity of the proteasome. PSI induced well characterized morphological changes in all cell lines studied, including vacuolization and accumulation of perinuclear aggregates, as detected by amido black staining. Together with previous reports on HeLa cells, the present findings support the hypothesis that ubiquitin- and proteasome-dependent proteolysis does not occurr randomly in the entire cell, but it is concentrated in a well defined perinuclear region, the proteolytic center.

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