An experimental model for improving fat graft viability and purity

Justin H. Piasecki, Karol A. Gutowski, Garet Lahvis, Katherine I. Moreno

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

BACKGROUND: Autologous fat is an excellent soft-tissue filler, given its abundance, ease of harvest, and natural appearance. However, graft longevity is unpredictable and is reported in the literature to be between 3 months and 8 years. METHODS: A genetically identical, age- and sex-matched mouse experiment was used to develop a model. Inguinal fat pads were subjected to different harvest and preparatory techniques. Primary endpoints-viability and purity-were assessed with the trypan blue viability assay and component counting with a hemocytometer. RESULTS: Viability and purity were highest after excisional harvest versus blunt or needle harvest, presumably secondary to differences in cellular trauma. Saline wash or centrifugation after harvest produced modest but statistically significant improvements in viability and purity. However, if grafts harvested in any fashion were treated with an initial collagenase digestion followed by an idealized centrifugation regimen and a single wash step, viability and purity were consistently 96 percent and 93 percent, respectively. CONCLUSIONS: Using an in vitro murine model, the authors have systematically developed a clinically practical model for creating a pure single-cell suspension of viable adipocytes that is reproducible, regardless of tissue harvest method.

Original languageEnglish (US)
Pages (from-to)1571-1583
Number of pages13
JournalPlastic and Reconstructive Surgery
Volume119
Issue number5
DOIs
StatePublished - Apr 2007
Externally publishedYes

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Centrifugation
Theoretical Models
Fats
Transplants
Trypan Blue
Groin
Collagenases
Adipocytes
Needles
Adipose Tissue
Digestion
Suspensions
Wounds and Injuries
In Vitro Techniques

ASJC Scopus subject areas

  • Surgery

Cite this

An experimental model for improving fat graft viability and purity. / Piasecki, Justin H.; Gutowski, Karol A.; Lahvis, Garet; Moreno, Katherine I.

In: Plastic and Reconstructive Surgery, Vol. 119, No. 5, 04.2007, p. 1571-1583.

Research output: Contribution to journalArticle

Piasecki, Justin H. ; Gutowski, Karol A. ; Lahvis, Garet ; Moreno, Katherine I. / An experimental model for improving fat graft viability and purity. In: Plastic and Reconstructive Surgery. 2007 ; Vol. 119, No. 5. pp. 1571-1583.
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