Abstract
Using a chemical genetics screen, we have identified ent-15-oxokaurenoic acid (EKA) as a chemical that causes prolonged mitotic arrest at a stage resembling prometaphase. EKA inhibits the association of the mitotic motor protein centromeric protein E with kinetochores and inhibits chromosome movement. Unlike most antimitotic agents, EKA does not inhibit the polymerization or depolymerization of tubulin. To identify EKA-interacting proteins, we used a cell-permeable biotinylated form that retains biological activity to isolate binding proteins from living cells. Mass spectrometric analysis identified six EKA-binding proteins, including Ran-binding protein 2, a kinetochore protein whose depletion by small interfering RNA causes a similar mitotic arrest phenotype.
Original language | English (US) |
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Pages (from-to) | 443-450 |
Number of pages | 8 |
Journal | ACS chemical biology |
Volume | 1 |
Issue number | 7 |
DOIs | |
State | Published - Aug 22 2006 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine