An endothelin-1 induced model of optic nerve ischemia in the rabbit

Selim Orgül, George A. Cioffi, David Wilson, David R. Bacon, E. Michael Van Buskirk

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Purpose. To evaluate blood flow reduction and topographic optic nerve changes after the local administration of endothelin-1 in vivo, delivered to the perineural region of the anterior optic nerve in the rabbit. Methods. Endothelin-1 (five rabbits) in a dosage of 0.1 μg/day or balanced salt solution (two rabbits) was delivered to the perineural region of the anterior optic nerve with osmotically driven minipumps. Optic nerve blood flow was determined by the colored microspheres technique after 14 days of local endothelin-1 or balanced salt solution administration to the microvasculature of the optic nerve. In addition, optic nerve blood flow was determined in two rabbits that had no minipump implants. The morphologic changes induced by reduction of blood flow were assessed in five additional rabbits implanted with osmotically driven minipumps containing endothelin-1 (0.1 μg/day). These rabbits were observed for 8 weeks, and the morphologic optic nerve changes were monitored with a confocal scantling laser ophthal moscope. Results. Independent of intraocular pressure, endothelin-1 induced a decrease in blood flow of approximately 38% in the experimental eye, compared to the decrease induced by balanced salt solution or to the decrease in rabbits without minipumps (analysis of covariance, P = 0.0092). Multivariate statistical analysis showed a significant change in topometric parameters (cup area, cup depth, rim volume) obtained with a confocal scanning laser ophthalmoscope, indicating an increase in optic nerve cupping and a decrease of the perineural rim volume in the experimental eyes (P = 0.017). Conclusions. The current results suggest that morphologic optic nerve alterations can be induced experimentally in the rabbit model after ischemia produced by the local administration of endothelin-1 to the perineural region of the anterior optic nerve.

Original languageEnglish (US)
Pages (from-to)1860-1869
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number9
StatePublished - Aug 1996

Fingerprint

Ischemic Optic Neuropathy
Endothelin-1
Optic Nerve
Rabbits
Salts
Lasers
Ophthalmoscopes
Microvessels
Intraocular Pressure
Microspheres
Multivariate Analysis
Ischemia

Keywords

  • blood flow
  • demyelination
  • glaucoma posterior segment
  • optic nerve
  • scanning laser

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Orgül, S., Cioffi, G. A., Wilson, D., Bacon, D. R., & Van Buskirk, E. M. (1996). An endothelin-1 induced model of optic nerve ischemia in the rabbit. Investigative Ophthalmology and Visual Science, 37(9), 1860-1869.

An endothelin-1 induced model of optic nerve ischemia in the rabbit. / Orgül, Selim; Cioffi, George A.; Wilson, David; Bacon, David R.; Van Buskirk, E. Michael.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 9, 08.1996, p. 1860-1869.

Research output: Contribution to journalArticle

Orgül, S, Cioffi, GA, Wilson, D, Bacon, DR & Van Buskirk, EM 1996, 'An endothelin-1 induced model of optic nerve ischemia in the rabbit', Investigative Ophthalmology and Visual Science, vol. 37, no. 9, pp. 1860-1869.
Orgül, Selim ; Cioffi, George A. ; Wilson, David ; Bacon, David R. ; Van Buskirk, E. Michael. / An endothelin-1 induced model of optic nerve ischemia in the rabbit. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 9. pp. 1860-1869.
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AB - Purpose. To evaluate blood flow reduction and topographic optic nerve changes after the local administration of endothelin-1 in vivo, delivered to the perineural region of the anterior optic nerve in the rabbit. Methods. Endothelin-1 (five rabbits) in a dosage of 0.1 μg/day or balanced salt solution (two rabbits) was delivered to the perineural region of the anterior optic nerve with osmotically driven minipumps. Optic nerve blood flow was determined by the colored microspheres technique after 14 days of local endothelin-1 or balanced salt solution administration to the microvasculature of the optic nerve. In addition, optic nerve blood flow was determined in two rabbits that had no minipump implants. The morphologic changes induced by reduction of blood flow were assessed in five additional rabbits implanted with osmotically driven minipumps containing endothelin-1 (0.1 μg/day). These rabbits were observed for 8 weeks, and the morphologic optic nerve changes were monitored with a confocal scantling laser ophthal moscope. Results. Independent of intraocular pressure, endothelin-1 induced a decrease in blood flow of approximately 38% in the experimental eye, compared to the decrease induced by balanced salt solution or to the decrease in rabbits without minipumps (analysis of covariance, P = 0.0092). Multivariate statistical analysis showed a significant change in topometric parameters (cup area, cup depth, rim volume) obtained with a confocal scanning laser ophthalmoscope, indicating an increase in optic nerve cupping and a decrease of the perineural rim volume in the experimental eyes (P = 0.017). Conclusions. The current results suggest that morphologic optic nerve alterations can be induced experimentally in the rabbit model after ischemia produced by the local administration of endothelin-1 to the perineural region of the anterior optic nerve.

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KW - demyelination

KW - glaucoma posterior segment

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KW - scanning laser

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