An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins

Stefan Jansson, Jenny Andersson, Soo Jung Kim, Grzegorz Jackowski

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predicted to contain 69 amino acids, to form one membrane-spanning α-helix and to have its N-terminus at the stromal side of the thylakoid membrane. The protein showed similarity to the LHC, ELIP and PsbS proteins of higher plants, but more pronounced to the high-light-inducible proteins (HLIPs) of cyanobacteria and red algae, to which no homologue previously has been detected in higher plants. As for HLIP and ELIP, high light increases the mRNA levels of the corresponding gene. Sequence comparisons indicate that the protein may bind chlorophyll and form dimers in the thylakoid membrane. The level of expression of the protein seems to be far lower than that of normal PSI and PSII subunits.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalPlant Molecular Biology
Volume42
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Arabidopsis Proteins
Arabidopsis thaliana
Thylakoids
Light
thylakoids
Proteins
proteins
Membranes
Rhodophyta
Amino Acids
Plant Proteins
amino acids
Chloroplasts
Chlorophyll
Arabidopsis
Algae
Dimers
Complementary DNA
Clone Cells
Cyanobacteria

Keywords

  • Arabidopsis thaliana
  • ELIP
  • EST
  • High light
  • HLIP
  • LHC protein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins. / Jansson, Stefan; Andersson, Jenny; Kim, Soo Jung; Jackowski, Grzegorz.

In: Plant Molecular Biology, Vol. 42, No. 2, 2000, p. 345-351.

Research output: Contribution to journalArticle

Jansson, Stefan ; Andersson, Jenny ; Kim, Soo Jung ; Jackowski, Grzegorz. / An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins. In: Plant Molecular Biology. 2000 ; Vol. 42, No. 2. pp. 345-351.
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