TY - JOUR
T1 - Amphetamine administration does not alter protein levels of the GLT-1 and EAAC1 glutamate transporter subtypes in rat midbrain, nucleus accumbens, striatum, or prefrontal cortex
AU - Sidiropoulou, Kyriaki
AU - Chao, Steven
AU - Lu, Wenxiao
AU - Wolf, Marina E.
N1 - Funding Information:
Supported by USPHS Grants DA09621, DA13006 and Independent Scientist Award DA00453 (M.E.W.). K.S. was supported by an Alexander S. Onassis Public Benefit Foundation Fellowship. S.Z.C. was supported by individual predoctoral National Research Service Award DA06024. We thank Dr. J.D. Rothstein (Johns Hopkins University) for providing an antibody to GLT-1 that was used in preliminary studies.
PY - 2001/6/20
Y1 - 2001/6/20
N2 - Our laboratory and others have previously shown that glutamate transmission is required for chronic amphetamine-induced neuroadaptations, and that glutamate transmission itself is altered by chronic amphetamine administration. For example, N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptor subunit expression are altered in a region- and withdrawal-specific manner. The goal of this study was to determine whether repeated amphetamine administration influences the expression of two glutamate transporter subtypes, GLT-1 and EAAC1. Rats were treated with saline or 5 mg/kg amphetamine for 5 days (chronic saline and amphetamine groups, respectively), or saline for 4 days and 5 mg/kg amphetamine on day 5 (acute amphetamine group), and decapitated 24 h after the last injection. Tissue was dissected from brain regions involved in the psychomotor effects of amphetamine (nucleus accumbens, striatum, prefrontal cortex, ventral tegmental area, and substantia nigra). Levels of GLT-1 and EAAC1 were quantified by Western blotting and normalized to actin levels. We found no significant change in levels of GLT-1 or EAAC1 in response to either acute or chronic amphetamine treatment. These findings suggest that the transporter component of the glutamate system might not play a significant role in the alterations in glutamate transmission observed following repeated amphetamine administration.
AB - Our laboratory and others have previously shown that glutamate transmission is required for chronic amphetamine-induced neuroadaptations, and that glutamate transmission itself is altered by chronic amphetamine administration. For example, N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptor subunit expression are altered in a region- and withdrawal-specific manner. The goal of this study was to determine whether repeated amphetamine administration influences the expression of two glutamate transporter subtypes, GLT-1 and EAAC1. Rats were treated with saline or 5 mg/kg amphetamine for 5 days (chronic saline and amphetamine groups, respectively), or saline for 4 days and 5 mg/kg amphetamine on day 5 (acute amphetamine group), and decapitated 24 h after the last injection. Tissue was dissected from brain regions involved in the psychomotor effects of amphetamine (nucleus accumbens, striatum, prefrontal cortex, ventral tegmental area, and substantia nigra). Levels of GLT-1 and EAAC1 were quantified by Western blotting and normalized to actin levels. We found no significant change in levels of GLT-1 or EAAC1 in response to either acute or chronic amphetamine treatment. These findings suggest that the transporter component of the glutamate system might not play a significant role in the alterations in glutamate transmission observed following repeated amphetamine administration.
KW - Behavioral sensitization
KW - Glutamate transmisssion
KW - Neuronal plasticity
KW - Stimulant
KW - Substantia nigra
KW - Ventral tegmental area
UR - http://www.scopus.com/inward/record.url?scp=0035919130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035919130&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(01)00110-3
DO - 10.1016/S0169-328X(01)00110-3
M3 - Article
C2 - 11406296
AN - SCOPUS:0035919130
SN - 0169-328X
VL - 90
SP - 187
EP - 192
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -