TY - JOUR
T1 - Alternatively spliced p53 RNA in transformed and normal cells of different tissue types
AU - Han, Kyung an
AU - Kulesz-martin, Molly F.
N1 - Funding Information:
We are grateful to Dr. Charles Wenner for 10T1/2 cells, Barbara Lisafeld for cell culture assistance and Dr. Bruce Dolnick for helpful discussion. This work was supported by National Institutes of Health grants CA31101, Biomedical Research Support Grant S07 RR05648-23 and CA16056.
PY - 1992/4/25
Y1 - 1992/4/25
N2 - The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast cells, mouse liver and testis suggests that this alternative splicing may be universal. The level of alternatively spliced p53 RNA was increased coordinatety with that of regularly spliced p53 in 10T1/2 cells in response to epidermal growth factor. Immunoprecipitation analysis of epidermal cells using monoclonal antibodies which recognize different epitopes of p53 suggested that distinct p53 proteins may be translated from both RNA species. Considering previous observations on the potential importance of carboxyl terminal sequences in p53 function, knowledge of the ubiquitous presence of alternatively spliced p53 is important for future studies of p53 function in normal cells and in oncogenesis.
AB - The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast cells, mouse liver and testis suggests that this alternative splicing may be universal. The level of alternatively spliced p53 RNA was increased coordinatety with that of regularly spliced p53 in 10T1/2 cells in response to epidermal growth factor. Immunoprecipitation analysis of epidermal cells using monoclonal antibodies which recognize different epitopes of p53 suggested that distinct p53 proteins may be translated from both RNA species. Considering previous observations on the potential importance of carboxyl terminal sequences in p53 function, knowledge of the ubiquitous presence of alternatively spliced p53 is important for future studies of p53 function in normal cells and in oncogenesis.
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U2 - 10.1093/nar/20.8.1979
DO - 10.1093/nar/20.8.1979
M3 - Article
C2 - 1579500
AN - SCOPUS:0026533125
SN - 0305-1048
VL - 20
SP - 1979
EP - 1981
JO - Nucleic acids research
JF - Nucleic acids research
IS - 8
ER -