Alternatively spliced p53 RNA in transformed and normal cells of different tissue types

Kyung An Han, Molly Kulesz-Martin

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast cells, mouse liver and testis suggests that this alternative splicing may be universal. The level of alternatively spliced p53 RNA was increased coordinately with that of regularly spliced p53 in 10T1/2 cells in response to epidermal growth factor, Immunoprecipitation analysis of epidermal cells using monoclonal antibodies which recognize different epitopes of p53 suggested that distinct p53 proteins may be translated from both RNA species. Considering previous observations on the potential importance of carboxyl terminal sequences in p53 function, knowledge of the ubiquitous presence of alternatively spliced p53 is important for future studies of p53 function in normal cells and in oncogenesis.

Original languageEnglish (US)
Pages (from-to)1979-1981
Number of pages3
JournalNucleic Acids Research
Volume20
Issue number8
StatePublished - 1992
Externally publishedYes

Fingerprint

RNA
Tissue
Cell
Cells
Epitopes
Alternative Splicing
Monoclonal antibodies
Monoclonal Antibody
Fibroblasts
Growth Factors
Factor Analysis
Tumor Suppressor Genes
Immunoprecipitation
Epidermal Growth Factor
Liver
Introns
Testis
Mouse
Tumors
Tumor

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Alternatively spliced p53 RNA in transformed and normal cells of different tissue types. / Han, Kyung An; Kulesz-Martin, Molly.

In: Nucleic Acids Research, Vol. 20, No. 8, 1992, p. 1979-1981.

Research output: Contribution to journalArticle

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