Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity

Arno Müllbacher, Ann Hill, Robert V. Blanden, William B. Cowden, Nicolas J C King, Ron Tha Hla

Research output: Contribution to journalArticle

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Abstract

In this report, experiments are described to differentiate between three potential models of class I MHC allorecognition, namely 1) recognition of peptide-free MHC, 2) peptide-MHC-specific recognition, and 3) peptide-MHC-nonspecific recognition. Using a nucleoprotein peptide (NPP) with a sequence derived from influenza virus nucleoprotein with high affinity for Kd class I MHC molecules, it is shown that target cells rapidly become lysable by Kd-NPP self-restricted cytotoxic T (Tc) cells, and retain sufficient Kd-NPP complexes for at least 72 h. Kd-specific alloreactive Tc cells at the clonal and polyclonal level do not show decreased lysis of Kd-bearing targets in the continuous long term (48 h) presence of NPP. Kd-stimulator cells modified with NPP are able to induce potent Kd-NPP-specific self-restricted Tc cells, however Kd-NPP stimulator cells do not generate Kd-NPP specific alloreactive Tc cells from CBA and B10.A (5R) mouse strains as tested by limiting dilution split clone experiments. Human cells infected with the vaccinia virus recombinant coding for the murine Kd class I MHC Ag can be lysed by murine Kd-specific alloreactive Tc cells. In addition the rate of reemergence of alloreactive and self-restricted Tc cell epitopes on virally infected target cells that had their cell-surface class I MHC Ag removed is identical. These results are consistent with model 3 namely that the majority of Tc precursor and effector cells recognize class I MHC Ag without peptide specificity.

Original languageEnglish (US)
Pages (from-to)1765-1772
Number of pages8
JournalJournal of Immunology
Volume147
Issue number6
StatePublished - Sep 15 1991
Externally publishedYes

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Histocompatibility Antigens Class I
Nucleoproteins
T-Lymphocytes
Peptides
Vaccinia virus
Orthomyxoviridae
Epitopes

ASJC Scopus subject areas

  • Immunology

Cite this

Müllbacher, A., Hill, A., Blanden, R. V., Cowden, W. B., King, N. J. C., & Hla, R. T. (1991). Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity. Journal of Immunology, 147(6), 1765-1772.

Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity. / Müllbacher, Arno; Hill, Ann; Blanden, Robert V.; Cowden, William B.; King, Nicolas J C; Hla, Ron Tha.

In: Journal of Immunology, Vol. 147, No. 6, 15.09.1991, p. 1765-1772.

Research output: Contribution to journalArticle

Müllbacher, A, Hill, A, Blanden, RV, Cowden, WB, King, NJC & Hla, RT 1991, 'Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity', Journal of Immunology, vol. 147, no. 6, pp. 1765-1772.
Müllbacher A, Hill A, Blanden RV, Cowden WB, King NJC, Hla RT. Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity. Journal of Immunology. 1991 Sep 15;147(6):1765-1772.
Müllbacher, Arno ; Hill, Ann ; Blanden, Robert V. ; Cowden, William B. ; King, Nicolas J C ; Hla, Ron Tha. / Alloreactive cytotoxic T cells recognize MHC class I antigen without peptide specificity. In: Journal of Immunology. 1991 ; Vol. 147, No. 6. pp. 1765-1772.
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