Allelotype of human malignant astrocytoma

Dan Fults, Carolyn A. Pedone, Gregory Thomas, Ray White

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Astrocytoma, the most common brain tumor in humans, is usually malignant and virtually incurable. Two types of malignant astrocytomas can be distinguished histopathologically: anaplastic astrocytoma and glioblastoma multiforme. Studies using DNA markers that detect restriction fragment length polymorphisms have shown that loci on chromosomes 10 and 17p are lost frequently in tumor DNA from malignant astrocytoma patients, suggesting that tumor suppressor genes important in astrocytoma tumorigenesis may be present on 2 different chromosomes. To identify additional regions of chromosome loss, we carried out an allelotype analysis of 41 malignant astrocytoma patients using restriction fragment length polymorphism markers for each arm of every human autosome. Loss of heterozygosity was found for every autosome except chromosome 21, indicating an even greater complexity of genomic alterations than reported previously. Many tumors showed loss of heterozygosity for multiple chromosomes and the number of chromosomes involved correlated with tumor histopathology. A high-resolution restriction fragment length polymorphism study of chromosome 10 loci in these patients showed that loss of broad regions of chromosome 10 was a common event, particularly in glioblastoma multiforme. An allelotype analysis has been carried out on only one other tumor, human colorectal carcinoma. Different profiles of allele loss were observed in malignant astrocytoma and colorectal carcinoma, suggesting that the genetic events leading to these 2 human cancers may proceed along different pathways.

Original languageEnglish (US)
Pages (from-to)5784-5789
Number of pages6
JournalCancer Research
Volume50
Issue number18
StatePublished - Sep 15 1990
Externally publishedYes

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Astrocytoma
Chromosomes, Human, Pair 10
Restriction Fragment Length Polymorphisms
Chromosomes
Loss of Heterozygosity
Neoplasms
Glioblastoma
Colorectal Neoplasms
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 2
Tumor Suppressor Genes
Genetic Markers
Brain Neoplasms
Carcinogenesis
Alleles
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fults, D., Pedone, C. A., Thomas, G., & White, R. (1990). Allelotype of human malignant astrocytoma. Cancer Research, 50(18), 5784-5789.

Allelotype of human malignant astrocytoma. / Fults, Dan; Pedone, Carolyn A.; Thomas, Gregory; White, Ray.

In: Cancer Research, Vol. 50, No. 18, 15.09.1990, p. 5784-5789.

Research output: Contribution to journalArticle

Fults, D, Pedone, CA, Thomas, G & White, R 1990, 'Allelotype of human malignant astrocytoma', Cancer Research, vol. 50, no. 18, pp. 5784-5789.
Fults D, Pedone CA, Thomas G, White R. Allelotype of human malignant astrocytoma. Cancer Research. 1990 Sep 15;50(18):5784-5789.
Fults, Dan ; Pedone, Carolyn A. ; Thomas, Gregory ; White, Ray. / Allelotype of human malignant astrocytoma. In: Cancer Research. 1990 ; Vol. 50, No. 18. pp. 5784-5789.
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