Albumin microbubble persistence during myocardial contrast echocardiography is associated with microvascular endothelial glycocalyx damage

Jonathan Lindner, Suad Ismail, William D. Spotnitz, Danny M. Skyba, Ananda R. Jayaweera, Sanjiv Kaul

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Background - We hypothesized that the persistence of albumin microbubbles within the myocardium during crystalloid cardioplegia (CP) infusion and ischemia-reperfusion (I-R) occurs because of endothelial injury. Methods and Results - The myocardial transit rate of albumin microbubbles was measured in 18 dogs perfused with different CP solutions and in 12 dogs undergoing I-R. Electron microscopy with cationized ferritin labeling of the glycocalyx was performed in 9 additional dogs after CP perfusion and in 3 additional dogs undergoing I-R. Microbubble transit was markedly prolonged during crystalloid CP perfusion. The addition of whole blood to the CP solution accelerated the transit rate in a dose-dependent fashion (P+ content, pH, PO2, osmolality, viscosity, and flow rate of the perfusate. Similarly, a proportion of microbubbles persisted in the myocardium after I-R, which was related to the duration of ischemia (P

Original languageEnglish (US)
Pages (from-to)2187-2194
Number of pages8
JournalCirculation
Volume98
Issue number20
StatePublished - Nov 17 1998
Externally publishedYes

Fingerprint

Glycocalyx
Microbubbles
Induced Heart Arrest
Echocardiography
Albumins
Dogs
Ischemia
Reperfusion
Myocardium
Perfusion
Viscosity
Osmolar Concentration
Electron Microscopy
Wounds and Injuries

Keywords

  • Echocardiography
  • Endothelium
  • Microspheres

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Albumin microbubble persistence during myocardial contrast echocardiography is associated with microvascular endothelial glycocalyx damage. / Lindner, Jonathan; Ismail, Suad; Spotnitz, William D.; Skyba, Danny M.; Jayaweera, Ananda R.; Kaul, Sanjiv.

In: Circulation, Vol. 98, No. 20, 17.11.1998, p. 2187-2194.

Research output: Contribution to journalArticle

Lindner, Jonathan ; Ismail, Suad ; Spotnitz, William D. ; Skyba, Danny M. ; Jayaweera, Ananda R. ; Kaul, Sanjiv. / Albumin microbubble persistence during myocardial contrast echocardiography is associated with microvascular endothelial glycocalyx damage. In: Circulation. 1998 ; Vol. 98, No. 20. pp. 2187-2194.
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