AIF and endoG translocation in noise exposure induced hair cell death

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Activation of caspases is a key element in the apoptotic process. However, mitochondria also play an important role via the release of proapoptotic proteins. This study investigated the roles of mitochondria-related apoptosis inducing factor (AIF) and endonuclease G (endoG), mitochondrion-specific nucleases, as well as caspase-3, an important mediator of apoptosis, in noise exposure induced hair cell death. Guinea pigs were exposed for 4 h/day to broadband noise at 122 dB SPL for 2 days. After the noise exposure, the cochleae were examined for the activity of caspase-3 with carboxyfluorescein-labeled fluoromethyl ketone (FMK)-peptide inhibitors. The cochleae were further examined for AIF and endoG translocation from the mitochondria by immunohistochemistry. Noise exposure triggered activation of caspase-3 in apoptotic hair cells. In the normal organ of Corti, AIF and endoG were co-localized to the mitochondria. After noise exposure, AIF translocated into the nuclei of apoptotic and necrotic hair cells. The translocation of endoG from mitochondria into the nucleus was also found in apoptotic OHCs. These findings indicate that mitochondria-released proapoptotic proteins, AIF and endoG, are important factors in a noise-induced hair cell death pathway.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalHearing Research
Volume211
Issue number1-2
DOIs
StatePublished - Jan 2006

Fingerprint

Apoptosis Inducing Factor
Noise
Mitochondria
Cell Death
Caspase 3
Cochlea
Organ of Corti
Caspases
endonuclease G
Ketones
Guinea Pigs
Proteins
Immunohistochemistry
Apoptosis
Peptides

Keywords

  • Apoptosis
  • Apoptosis inducing factor
  • Caspase
  • Endonuclease G
  • Guinea pig
  • Mitochondria
  • Noise exposure

ASJC Scopus subject areas

  • Sensory Systems

Cite this

AIF and endoG translocation in noise exposure induced hair cell death. / Han, Weiju; Shi, Xiao; Nuttall, Alfred.

In: Hearing Research, Vol. 211, No. 1-2, 01.2006, p. 85-95.

Research output: Contribution to journalArticle

@article{9a269a3186da4bc78a78e0f14028eea8,
title = "AIF and endoG translocation in noise exposure induced hair cell death",
abstract = "Activation of caspases is a key element in the apoptotic process. However, mitochondria also play an important role via the release of proapoptotic proteins. This study investigated the roles of mitochondria-related apoptosis inducing factor (AIF) and endonuclease G (endoG), mitochondrion-specific nucleases, as well as caspase-3, an important mediator of apoptosis, in noise exposure induced hair cell death. Guinea pigs were exposed for 4 h/day to broadband noise at 122 dB SPL for 2 days. After the noise exposure, the cochleae were examined for the activity of caspase-3 with carboxyfluorescein-labeled fluoromethyl ketone (FMK)-peptide inhibitors. The cochleae were further examined for AIF and endoG translocation from the mitochondria by immunohistochemistry. Noise exposure triggered activation of caspase-3 in apoptotic hair cells. In the normal organ of Corti, AIF and endoG were co-localized to the mitochondria. After noise exposure, AIF translocated into the nuclei of apoptotic and necrotic hair cells. The translocation of endoG from mitochondria into the nucleus was also found in apoptotic OHCs. These findings indicate that mitochondria-released proapoptotic proteins, AIF and endoG, are important factors in a noise-induced hair cell death pathway.",
keywords = "Apoptosis, Apoptosis inducing factor, Caspase, Endonuclease G, Guinea pig, Mitochondria, Noise exposure",
author = "Weiju Han and Xiao Shi and Alfred Nuttall",
year = "2006",
month = "1",
doi = "10.1016/j.heares.2005.10.004",
language = "English (US)",
volume = "211",
pages = "85--95",
journal = "Hearing Research",
issn = "0378-5955",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - AIF and endoG translocation in noise exposure induced hair cell death

AU - Han, Weiju

AU - Shi, Xiao

AU - Nuttall, Alfred

PY - 2006/1

Y1 - 2006/1

N2 - Activation of caspases is a key element in the apoptotic process. However, mitochondria also play an important role via the release of proapoptotic proteins. This study investigated the roles of mitochondria-related apoptosis inducing factor (AIF) and endonuclease G (endoG), mitochondrion-specific nucleases, as well as caspase-3, an important mediator of apoptosis, in noise exposure induced hair cell death. Guinea pigs were exposed for 4 h/day to broadband noise at 122 dB SPL for 2 days. After the noise exposure, the cochleae were examined for the activity of caspase-3 with carboxyfluorescein-labeled fluoromethyl ketone (FMK)-peptide inhibitors. The cochleae were further examined for AIF and endoG translocation from the mitochondria by immunohistochemistry. Noise exposure triggered activation of caspase-3 in apoptotic hair cells. In the normal organ of Corti, AIF and endoG were co-localized to the mitochondria. After noise exposure, AIF translocated into the nuclei of apoptotic and necrotic hair cells. The translocation of endoG from mitochondria into the nucleus was also found in apoptotic OHCs. These findings indicate that mitochondria-released proapoptotic proteins, AIF and endoG, are important factors in a noise-induced hair cell death pathway.

AB - Activation of caspases is a key element in the apoptotic process. However, mitochondria also play an important role via the release of proapoptotic proteins. This study investigated the roles of mitochondria-related apoptosis inducing factor (AIF) and endonuclease G (endoG), mitochondrion-specific nucleases, as well as caspase-3, an important mediator of apoptosis, in noise exposure induced hair cell death. Guinea pigs were exposed for 4 h/day to broadband noise at 122 dB SPL for 2 days. After the noise exposure, the cochleae were examined for the activity of caspase-3 with carboxyfluorescein-labeled fluoromethyl ketone (FMK)-peptide inhibitors. The cochleae were further examined for AIF and endoG translocation from the mitochondria by immunohistochemistry. Noise exposure triggered activation of caspase-3 in apoptotic hair cells. In the normal organ of Corti, AIF and endoG were co-localized to the mitochondria. After noise exposure, AIF translocated into the nuclei of apoptotic and necrotic hair cells. The translocation of endoG from mitochondria into the nucleus was also found in apoptotic OHCs. These findings indicate that mitochondria-released proapoptotic proteins, AIF and endoG, are important factors in a noise-induced hair cell death pathway.

KW - Apoptosis

KW - Apoptosis inducing factor

KW - Caspase

KW - Endonuclease G

KW - Guinea pig

KW - Mitochondria

KW - Noise exposure

UR - http://www.scopus.com/inward/record.url?scp=30944466820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30944466820&partnerID=8YFLogxK

U2 - 10.1016/j.heares.2005.10.004

DO - 10.1016/j.heares.2005.10.004

M3 - Article

C2 - 16309861

AN - SCOPUS:30944466820

VL - 211

SP - 85

EP - 95

JO - Hearing Research

JF - Hearing Research

SN - 0378-5955

IS - 1-2

ER -