Age-related changes in susceptibility of rat brain slice cultures including hippocampus to encephalomyocarditis virus

Weiping Su, Aito Ueno-Yamanouchi, Koji Uetsuka, Hiroyuki Nakayama, Kunio Doi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Replication of the D variant of encephalomyocarditis virus (EMC-D) and its cytopathic effects were studied in the brain slice cultures including hippocampus (hippocampal slice) obtained from postnatal 1-, 4-, 7-, 14-, 28- and 56-day-old Fischer 344 rats. At 0, 12, 24, 36 and 48 h after infection, virus titres of the slices and culture media were assayed. Viral replication was observed in cultures from 1-to 28-day-old rats, and the highest titre was recorded in the slice and culture medium from the youngest rat. The peak of virus titre decreased with age and no distinct viral replication was observed in the cultures from 56-day-old rats. Light microscopy revealed that degenerative and necrotic changes appeared in the infected hippocampal slices from 1 to 28-day-old rats, and the changes became less prominent with age. In situ hybridization and indirect immunofluorescence staining showed that positive signals of viral RNA and antigen were prominent in younger rats and decreased with age. These results suggest that an age-related decrease in the susceptibility of rat brain to EMC-D is less related to the maturation of the immune system but possibly to that of the neurone.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalInternational Journal of Experimental Pathology
Volume80
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Encephalomyocarditis virus
Hippocampus
Brain
Viral Load
Culture Media
Viral Antigens
Inbred F344 Rats
Viral RNA
Indirect Fluorescent Antibody Technique
In Situ Hybridization
Microscopy
Immune System
Staining and Labeling
Neurons
Light
Infection

Keywords

  • Age-related
  • Encephalomyocarditis virus
  • In situ hybridization
  • Organotypic cultures
  • Rat

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Age-related changes in susceptibility of rat brain slice cultures including hippocampus to encephalomyocarditis virus. / Su, Weiping; Ueno-Yamanouchi, Aito; Uetsuka, Koji; Nakayama, Hiroyuki; Doi, Kunio.

In: International Journal of Experimental Pathology, Vol. 80, No. 6, 1999, p. 349-355.

Research output: Contribution to journalArticle

Su, Weiping ; Ueno-Yamanouchi, Aito ; Uetsuka, Koji ; Nakayama, Hiroyuki ; Doi, Kunio. / Age-related changes in susceptibility of rat brain slice cultures including hippocampus to encephalomyocarditis virus. In: International Journal of Experimental Pathology. 1999 ; Vol. 80, No. 6. pp. 349-355.
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