Abstract
Replication of the D variant of encephalomyocarditis virus (EMC-D) and its cytopathic effects were studied in the brain slice cultures including hippocampus (hippocampal slice) obtained from postnatal 1-, 4-, 7-, 14-, 28- and 56-day-old Fischer 344 rats. At 0, 12, 24, 36 and 48 h after infection, virus titres of the slices and culture media were assayed. Viral replication was observed in cultures from 1-to 28-day-old rats, and the highest titre was recorded in the slice and culture medium from the youngest rat. The peak of virus titre decreased with age and no distinct viral replication was observed in the cultures from 56-day-old rats. Light microscopy revealed that degenerative and necrotic changes appeared in the infected hippocampal slices from 1 to 28-day-old rats, and the changes became less prominent with age. In situ hybridization and indirect immunofluorescence staining showed that positive signals of viral RNA and antigen were prominent in younger rats and decreased with age. These results suggest that an age-related decrease in the susceptibility of rat brain to EMC-D is less related to the maturation of the immune system but possibly to that of the neurone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 349-355 |
| Number of pages | 7 |
| Journal | International Journal of Experimental Pathology |
| Volume | 80 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Age-related
- Encephalomyocarditis virus
- In situ hybridization
- Organotypic cultures
- Rat
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Age-related changes in susceptibility of rat brain slice cultures including hippocampus to encephalomyocarditis virus. / Su, Weiping; Ueno-Yamanouchi, Aito; Uetsuka, Koji; Nakayama, Hiroyuki; Doi, Kunio.
In: International Journal of Experimental Pathology, Vol. 80, No. 6, 1999, p. 349-355.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Age-related changes in susceptibility of rat brain slice cultures including hippocampus to encephalomyocarditis virus
AU - Su, Weiping
AU - Ueno-Yamanouchi, Aito
AU - Uetsuka, Koji
AU - Nakayama, Hiroyuki
AU - Doi, Kunio
PY - 1999
Y1 - 1999
N2 - Replication of the D variant of encephalomyocarditis virus (EMC-D) and its cytopathic effects were studied in the brain slice cultures including hippocampus (hippocampal slice) obtained from postnatal 1-, 4-, 7-, 14-, 28- and 56-day-old Fischer 344 rats. At 0, 12, 24, 36 and 48 h after infection, virus titres of the slices and culture media were assayed. Viral replication was observed in cultures from 1-to 28-day-old rats, and the highest titre was recorded in the slice and culture medium from the youngest rat. The peak of virus titre decreased with age and no distinct viral replication was observed in the cultures from 56-day-old rats. Light microscopy revealed that degenerative and necrotic changes appeared in the infected hippocampal slices from 1 to 28-day-old rats, and the changes became less prominent with age. In situ hybridization and indirect immunofluorescence staining showed that positive signals of viral RNA and antigen were prominent in younger rats and decreased with age. These results suggest that an age-related decrease in the susceptibility of rat brain to EMC-D is less related to the maturation of the immune system but possibly to that of the neurone.
AB - Replication of the D variant of encephalomyocarditis virus (EMC-D) and its cytopathic effects were studied in the brain slice cultures including hippocampus (hippocampal slice) obtained from postnatal 1-, 4-, 7-, 14-, 28- and 56-day-old Fischer 344 rats. At 0, 12, 24, 36 and 48 h after infection, virus titres of the slices and culture media were assayed. Viral replication was observed in cultures from 1-to 28-day-old rats, and the highest titre was recorded in the slice and culture medium from the youngest rat. The peak of virus titre decreased with age and no distinct viral replication was observed in the cultures from 56-day-old rats. Light microscopy revealed that degenerative and necrotic changes appeared in the infected hippocampal slices from 1 to 28-day-old rats, and the changes became less prominent with age. In situ hybridization and indirect immunofluorescence staining showed that positive signals of viral RNA and antigen were prominent in younger rats and decreased with age. These results suggest that an age-related decrease in the susceptibility of rat brain to EMC-D is less related to the maturation of the immune system but possibly to that of the neurone.
KW - Age-related
KW - Encephalomyocarditis virus
KW - In situ hybridization
KW - Organotypic cultures
KW - Rat
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UR - http://www.scopus.com/inward/citedby.url?scp=0033399161&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2613.1999.00133.x
DO - 10.1046/j.1365-2613.1999.00133.x
M3 - Article
C2 - 10632784
AN - SCOPUS:0033399161
VL - 80
SP - 349
EP - 355
JO - International Journal of Experimental Pathology
JF - International Journal of Experimental Pathology
SN - 0959-9673
IS - 6
ER -