Adhesion of platelets to surface-bound fibrinogen under flow

Tabish N. Zaidi, Larry V. McIntire, David Farrell, Perumal Thiagarajan

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

After platelet activation, fibrinogen mediates platelet-platelet interactions leading to platelet aggregation. In addition, fibrinogen can also function as a cell adhesion molecule, providing a substratum for adhesion of platelets and endothelial cells. In this report, we studied the adhesion of platelets to surface-immobilized fibrinogen under flow in different shear rates. Heparinized whole blood containing mepacrine-labeled platelets was perfused for two minutes at various wall shear rates from 250 to 2,000 s-1 in a parallel plate flow chamber. The number of adherent fluorescent platelets was quantitated every 15 seconds with an epifluorescent videomicroscope and digital image processing system. When compared with platelet adhesion and aggregation seen on glass surfaces coated with type I bovine collagen, a significant increase in platelet adhesion was observed on immobilized fibrinogen up to wall shear rates of 800 s-1. The adherent platelets formed a single layer on fibrinogen-coated surfaces. Under identical conditions, no significant adhesion was observed on fibronectin- or vitronectin-coated surfaces. Although platelet adhesion to collagen was substantially inhibited by the platelet inhibitors prostaglandin E1 and theophylline, these inhibitors had no effect on platelet adhesion to fibrinogen. Platelets adhered to recombinant homodimeric wild-type (γ400- 411) fibrinogen, but not to the recombinant homodimeric γ' variant of fibrinogen. Platelet adhesion to recombinant fibrinogen with RGD to RGE mutations at positions α95-97 and α572-574 was similar to that with plasma- derived fibrinogen. These results show that platelets adhere to fibrinogen- coated surfaces under moderate wall shear rates, that the interaction is mediated by the fibrinogen 400-411 sequence at the carboxy-terminus of the γ chain, and that the interaction is independent of platelet activation and the RGD sequences in the α chain.

Original languageEnglish (US)
Pages (from-to)2967-2972
Number of pages6
JournalBlood
Volume88
Issue number8
StatePublished - Oct 15 1996
Externally publishedYes

Fingerprint

Platelets
Fibrinogen
Blood Platelets
Adhesion
Shear deformation
Platelet Activation
Platelet Aggregation
Vitronectin
Quinacrine
Collagen
Agglomeration
Chemical activation
Alprostadil
Platelet Aggregation Inhibitors
Cell Adhesion Molecules
Theophylline
Collagen Type I
Fibronectins
Glass
Endothelial cells

ASJC Scopus subject areas

  • Hematology

Cite this

Zaidi, T. N., McIntire, L. V., Farrell, D., & Thiagarajan, P. (1996). Adhesion of platelets to surface-bound fibrinogen under flow. Blood, 88(8), 2967-2972.

Adhesion of platelets to surface-bound fibrinogen under flow. / Zaidi, Tabish N.; McIntire, Larry V.; Farrell, David; Thiagarajan, Perumal.

In: Blood, Vol. 88, No. 8, 15.10.1996, p. 2967-2972.

Research output: Contribution to journalArticle

Zaidi, TN, McIntire, LV, Farrell, D & Thiagarajan, P 1996, 'Adhesion of platelets to surface-bound fibrinogen under flow', Blood, vol. 88, no. 8, pp. 2967-2972.
Zaidi TN, McIntire LV, Farrell D, Thiagarajan P. Adhesion of platelets to surface-bound fibrinogen under flow. Blood. 1996 Oct 15;88(8):2967-2972.
Zaidi, Tabish N. ; McIntire, Larry V. ; Farrell, David ; Thiagarajan, Perumal. / Adhesion of platelets to surface-bound fibrinogen under flow. In: Blood. 1996 ; Vol. 88, No. 8. pp. 2967-2972.
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