Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome

Yoram G. Weiss, Alina Maloyan, John Tazelaar, Nichelle Raj, Clifford S. Deutschman

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The acute respiratory distress syndrome (ARDS) provokes three pathologic processes: unchecked inflammation, interstitial/alveolar protein accumulation, and destruction of pulmonary epithelial cells. The highly conserved heat shock protein HSP-70 can limit all three responses but is not appropriately expressed in the lungs after cecal ligation and double puncture (2CLP), a clinically relevant model of ARDS. We hypothesize that restoring expression of HSP-70 using adenovirus-mediated gene therapy will limit pulmonary pathology following 2CLP. We administered a vector containing the porcine HSP-70 cDNA driven by a CMV promoter (AdHSP) into the lungs of rats subjected to 2CLP or sham operation. Administration of AdHSP after either sham operation or 2CLP increased HSP-70 protein expression in lung tissue, as determined by immunohistochemistry and Western blot hybridization. Administration of AdHSP significantly attenuated interstitial and alveolar edema and protein exudation and dramatically decreased neutrophil accumulation, relative to a control adenovirus. CLP-associated mortality at 48 hours was reduced by half. Modulation of HSP-70 production reduces pathologic changes and may improve outcome in experimental ARDS.

Original languageEnglish (US)
Pages (from-to)801-806
Number of pages6
JournalJournal of Clinical Investigation
Volume110
Issue number6
DOIs
StatePublished - Sep 2002
Externally publishedYes

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Adult Respiratory Distress Syndrome
Epithelium
Lung
Adenoviridae
Proteins
HSP70 Heat-Shock Proteins
Pathologic Processes
Punctures
Genetic Therapy
Ligation
Edema
Neutrophils
Swine
Complementary DNA
Western Blotting
Epithelial Cells
Immunohistochemistry
Pathology
Inflammation
Mortality

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome. / Weiss, Yoram G.; Maloyan, Alina; Tazelaar, John; Raj, Nichelle; Deutschman, Clifford S.

In: Journal of Clinical Investigation, Vol. 110, No. 6, 09.2002, p. 801-806.

Research output: Contribution to journalArticle

Weiss, Yoram G. ; Maloyan, Alina ; Tazelaar, John ; Raj, Nichelle ; Deutschman, Clifford S. / Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome. In: Journal of Clinical Investigation. 2002 ; Vol. 110, No. 6. pp. 801-806.
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